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DNA is inserted
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The three-dimensional (3D) organization of chromosomes is linked to epigenetic regulation and transcriptional activity. However, only few functional features of 3D chromatin architecture have been described to date.
15p
vigalileogalilei
27-02-2022
13
1
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Genomic imprinting is an epigenetic phenomenon that allows a subset of genes to be expressed mono-allelically based on the parent of origin and is typically regulated by differential DNA methylation inherited from gametes.
17p
vielonmusk
30-01-2022
7
1
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There is no effective way to detect structure variations (SVs) and extra-chromosomal circular DNAs (ecDNAs) at single-cell whole-genome level. Here, we develop a novel third-generation sequencing platform-based single-cell whole-genome sequencing (scWGS) method named SMOOTH-seq (single-molecule real-time sequencing of long fragments amplified through transposon insertion).
19p
viarchimedes
26-01-2022
14
0
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Expression screening of environmental DNA (eDNA) libraries is a popular approach for the identification and characterization of novel microbial enzymes with promising biotechnological properties. In such “functional metagenomics” experiments, inserts, selected on the basis of activity assays, are sequenced with high throughput sequencing technologies. Assembly is followed by gene prediction, annotation and identification of candidate genes that are subsequently evaluated for biotechnological applications.
10p
vilarryellison
29-10-2021
9
1
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Identification of mutations from next-generation sequencing data typically requires a balance between sensitivity and accuracy. This is particularly true of DNA insertions and deletions (indels), that can impart significant phenotypic consequences on cells but are harder to call than substitution mutations from whole genome mutation accumulation experiments.
19p
vibeauty
23-10-2021
11
1
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Genomic islands (GIs) are inserts of foreign DNA that have potentially arisen through horizontal gene transfer (HGT). There are evidences that GIs can contribute significantly to the evolution of prokaryotes. The acquisition of GIs through HGT in eukaryotes has, however, been largely unexplored. In this study, the previously developed GI prediction tool, SeqWord Gene Island Sniffer (SWGIS), is modified to predict GIs in eukaryotic chromosomes.
9p
vibeauty
23-10-2021
8
0
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Reporter methods to quantitatively measure the efficiency and specificity of genome editing tools are important for the development of novel editing techniques and successful applications of available ones. However, the existing methods have major limitations in sensitivity, accuracy, and/or readiness for in vivo applications.
10p
visilicon2711
20-08-2021
9
1
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The evolution of bacteria is shaped by different mechanisms such as mutation, gene deletion, duplication, or insertion of foreign DNA among others. These genetic changes can accumulate in the descendants as a result of natural selection.
14p
vijeeni2711
30-06-2021
9
1
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The preferred choice for molecular marker development is identifying existing variation in populations through DNA sequencing. With the genome resources currently available for bitter gourd (Momordica charantia), it is now possible to detect genome-wide insertion-deletion (InDel) polymorphisms among bitter gourd populations, which guides the efficient development of InDel markers.
9p
vilichoo2711
23-06-2021
10
1
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Genome editing is a technique in which changes are made in the DNA of any organism. A nuclease promotes breaks in the DNA at a specific sequence which is repaired by several mechanisms. It is one of the efficient technologies which enable us to change and edit genome of any organism precisely and accurately. Multiple genome editing technologies have been employed, including zinc finger nucleases, mega nucleases, clustered regularly interspaced short palindromic repeats (CRISPR) along with Cas9 protein and transcription activator-like effector nucleases to modify genome of an organism.
9p
trinhthamhodang1218
26-02-2021
13
3
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Barcode multiplexing is a key strategy for sharing the rising capacity of next-generation sequencing devices: Synthetic DNA tags, called barcodes, are attached to natural DNA fragments within the library preparation procedure. Different libraries, can individually be labeled with barcodes for a joint sequencing procedure.
14p
vikentucky2711
26-11-2020
14
0
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DNBSEQ™ platforms are new massively parallel sequencing (MPS) platforms that use DNA nanoball technology. Use of data generated from DNBSEQ™ platforms to detect single nucleotide variants (SNVs) and small insertions and deletions (indels) has proven to be quite effective, while the feasibility of copy number variants (CNVs) detection is unclear.
14p
vikentucky2711
24-11-2020
14
1
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Insertions and deletions (indels) account for more nucleotide differences between two related DNA sequences than substitutions do, and thus it is imperative to develop a method to reliably calculate the occurrence probabilities of sequence alignments via evolutionary processes on an entire sequence.
21p
vioklahoma2711
19-11-2020
18
2
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During evolution, genomes are modified by large scale structural events, such as rearrangements, deletions or insertions of large blocks of DNA. Of particular interest, in order to better understand how this type of genomic evolution happens, is the reconstruction of ancestral genomes, given a phylogenetic tree with extant genomes at its leaves.
14p
vioklahoma2711
19-11-2020
13
1
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Comparing sets of sequences is a situation frequently encountered in bioinformatics, examples being comparing an assembly to a reference genome, or two genomes to each other. The purpose of the comparison is usually to find where the two sets differ, e.g. to find where a subsequence is repeated or deleted, or where insertions have been introduced.
14p
viflorida2711
30-10-2020
14
2
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Histone acetyltransferase complex NuA4 and histone variant exchanging complex SWR1 are two chromatin modifying complexes which act cooperatively in yeast and share some intriguing structural similarities.
15p
viminato2711
22-05-2020
15
1
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Plant architecture, which is mostly determined by shoot branching, plays an important role in plant growth and development. Thus, it is essential to explore the regulatory molecular mechanism of branching patterns based on the economic and ecological importance.
16p
viharuno2711
21-04-2020
10
1
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PDX1.2 has recently been shown to be a regulator of vitamin B6 biosynthesis in plants and is implicated in biotic and abiotic stress resistance. PDX1.2 expression is strongly and rapidly induced by heat stress.
14p
viharuno2711
21-04-2020
5
0
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Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of interest.
15p
vihamax2711
21-04-2020
12
1
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Transposable elements, found in almost all organisms, are short sequences of DNA that have the ability to move from one location to the other locations in the genome. Transposons make up 10% of eukaryotic genomes. They provide a means for genomic change and variation, particularly in response to stress (McClintock’s stress hypothesis).There is no known example of transposon playing a normal role in development. They are called as selfish DNA and can be used as genetic markers, as mutagens for transposon tagging and isolation of gene, as transformation vectors and as cloning vehicle.
11p
chauchaungayxua4
18-03-2020
17
2
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