DNA loops
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The Tajima’s test indicated that the analysed population in balancing selection with a D-value of 2.39. A phylogenetic tree based on 455 bp of hypervariable mtDloop sequence classified 17 Bang Troi chicken individuals into three clades A, B, and E, which are common groups of Vietnamese and Asia indigenous chickens. This result provides the first genetic information and maternal lineages of Bang Troi chickens and it can support for registration of local chicken breeds.
6p dianmotminh02 03-05-2024 2 1 Download
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To study the effect of inhibitor of differentiation 3 (ID3) on radiotherapy in patients with rectal cancer and to explore its primary mechanism. Methods Cell proliferation and clonogenic assays were used to study the relationship between ID3 and radiosensitivity. Co-immunoprecipitation and immunofluorescence were performed to analyze the possible mechanism of ID3 in the radiosensitivity of colorectal cancer.
16p visharma 20-10-2023 8 3 Download
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Công nghệ khuếch đại đẳng nhiệt qua trung gian vòng lặp (Loop-mediated Isothermal Amplification - LAMP) là phương pháp khuếch đại các phân tử DNA đơn giản, hiệu quả được phát triển bởi Notomi và cộng sự vào năm 2000. Bài viết trình bày việc xây dựng quy trình biểu hiện và tinh sạch enzyme Br512 và đánh giá hoạt tính của enzyme, ứng dụng trong chẩn đoán virut HPV type 18.
5p viberbers 09-08-2023 5 2 Download
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Việc nghiên cứu những biến thể trên vùng D-loop ty thể của những người bệnh bị bệnh lý mạch vành vẫn chưa có những kết quả đầy đủ về mối liên quan. Nhằm cung cấp thêm những số liệu khoa học về biến thể trên người bệnh có bệnh lý mạch vành nhóm tác giả đã tiến hành Nghiên cứu đặc điểm trình tự nucleotide đặc hiệu gen HV2 trên vùng D-Loop ty thể của các người bệnh có bệnh lý mạch vành.
5p vianapatricia 22-06-2022 9 2 Download
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Co-transcriptional R-loops are abundant non-B DNA structures in mammalian genomes. DNA Topoisomerase I (Top1) is often thought to regulate R-loop formation owing to its ability to resolve both positive and negative supercoils. How Top1 regulates R-loop structures at a global level is unknown.
18p vigalileogalilei 27-02-2022 12 1 Download
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Chromatin loops form a basic unit of interphase nuclear organization, with chromatin loop anchor points providing contacts between regulatory regions and promoters. However, the mutational landscape at these anchor points remains under-studied. Here, we describe the unusual patterns of somatic mutations and germline variation associated with loop anchor points and explore the underlying features influencing these patterns.
14p vigalileogalilei 27-02-2022 15 1 Download
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CTCF binding to DNA helps partition the mammalian genome into discrete structural and regulatory domains. Complete removal of CTCF from mammalian cells causes catastrophic genome dysregulation, likely due to widespread collapse of 3D chromatin looping and alterations to inter- and intra-TAD interactions within the nucleus.
17p vigalileogalilei 27-02-2022 13 1 Download
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Việc phát hiện sớm và nhanh các mầm bệnh trên thực phẩm do vi khuẩn Salmonella gây ra sẽ giúp kiểm soát được thực phẩm bẩn trên thị trường. Vì vậy, trong nghiên cứu này đã xây dựng quy trình phát hiện Salmonella trong các loại thực phẩm bằng kỹ thuật LAMP để phát hiện nhanh và chính xác các chủng vi khuẩn Salmonella spp. dựa trên bộ mồi invA2 chuyên biệt cho vùng gen invA của vi khuẩn Salmonella.
10p vialexanderfleming 09-02-2022 35 3 Download
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Whole-genome bisulfite sequencing (WGBS) is the gold standard for studying landscape DNA methylation. Current computational methods for WGBS are mainly designed for gene regulatory regions with multiple under-methylated CpGs (UMCs), such as promoters and enhancers.
10p vialfrednobel 29-01-2022 10 0 Download
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Capture Hi-C (CHi-C) is a method for profiling chromosomal interactions involving targeted regions of interest, such as gene promoters, globally and at high resolution. Signal detection in CHi-C data involves a number of statistical challenges that are not observed when using other Hi-C-like techniques.
17p viaristotle 29-01-2022 4 0 Download
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Chromosome conformation capture (3C)-based techniques have revolutionized the field of nuclear organization, partly replacing DNA FISH as the method of choice for studying three-dimensional chromosome architecture. Although DNA FISH is commonly used for confirming 3C-based findings, the two techniques are conceptually and technically different and comparing their results is not trivial. Here, we discuss both 3C-based techniques and DNA FISH approaches to highlight their similarities and differences.
9p viaristotle 29-01-2022 11 0 Download
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The three-dimensional genome organization is critical for gene regulation and can malfunction in diseases like cancer. As a key regulator of genome organization, CCCTC-binding factor (CTCF) has been characterized as a DNA-binding protein with important functions in maintaining the topological structure of chromatin and inducing DNA looping.
30p viarchimedes 26-01-2022 3 0 Download
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Polyploidy is ubiquitous in eukaryotic plant and fungal lineages, and it leads to the co-existence of several copies of similar or related genomes in one nucleus. In plants, polyploidy is considered a major factor in successful domestication. H
20p viarchimedes 26-01-2022 11 0 Download
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Lung adenocarcinoma (LUAD) is the most common histological subtype of primary lung cancer. To identify the biomarker of diagnosis for LUAD is of great significance. Long non-coding RNAs (lncRNAs) were previously revealed to exert vital efects in numerous cancers.
12p vimackenziebezos 29-11-2021 7 1 Download
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Chromosomal architecture, which is constituted by chromatin loops, plays an important role in cellular functions. Gene expression and cell identity can be regulated by the chromatin loop, which is formed by proximal or distal enhancers and promoters in linear DNA (1D).
13p visilicon2711 20-08-2021 8 1 Download
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Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungal pathogen and the role of its colonization in pulmonary diseases has become a popular focus in recent years. The aim of this study was to develop a modified loop-mediated isothermal amplification (LAMP) assay for detection of Pneumocystis jirovecii (P. jirovecii) DNA amongst non-HIV patients with various pulmonary diseases and use it to examine the prevalence and assess the association of P. jirovecii colonization with clinical characteristics of these diseases.
13p vimaine2711 26-03-2021 11 2 Download
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Nghiên cứu đã sử dụng song song việc phân tích Bayesian và các bộ nghiên cứu mô phỏng để đánh giá sự khác biệt giữa chó bản địa và chó sói, tần số và phân bố lai tạo, lai có định hướng. Nghiên cứu thu được ba kết quả quan trọng: Không có sự khác biệt về di truyền giữa chó bản địa và chó sói hoang; giới hạn sinh sản và mở rộng phân bố của chó sói; bằng chứng về sự biến động trong quần thể lai được tạo ra bởi quá trình lai giữa chó bản địa đực và sói cái.
8p vipalau2711 04-01-2021 35 2 Download
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The molecular recognition based on the complementary base pairing of deoxyribonucleic acid (DNA) is the fundamental principle in the fields of genetics, DNA nanotechnology and DNA computing. We present an exhaustive DNA sequence design algorithm that allows to generate sets containing a maximum number of sequences with defined properties.
17p viwyoming2711 16-12-2020 15 1 Download
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A variety of DNA binding proteins are involved in regulating and shaping the packing of chromatin. They aid the formation of loops in the DNA that function to isolate different structural domains.
12p vikentucky2711 24-11-2020 11 1 Download
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DNA inside eukaryotic cells wraps around histones to form the 11nm chromatin fiber that can further fold into higher-order DNA loops, which may depend on the binding of architectural factors. Predicting how the DNA will fold given a distribution of bound factors, here viewed as a type of sequence, is currently an unsolved problem and several heterogeneous polymer models have shown that many features of the measured structure can be reproduced from simulations.
12p viconnecticut2711 28-10-2020 13 0 Download