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DsRed fluorescent reporter gene
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In this study, we report for the first time that the phleomycin resistance gene can be employed as a selectable marker for genetic transformation of P. digitatum using A. tumefaciens. Our work shows that the transformation efficiency with the phleomycin resistance marker could achieve over 1000 transformants for 106 spores.
6p
kethamoi7
15-08-2020
14
2
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Aspergillus oryzae, a safe filamentous fungus, is widely used in food and enzyme production. In this study, we examined a cultivation model using rice husks as carrier to assess the capacity of recombinant protein production in A. oryzae. The model was first tested with the A. oryzae strain expressing the DsRed reporter gene. The expression of DsRed was easily detected by the pink color of the fungal mycelium on culture media and under a fluorescence microscope. The model was then evaluated with the phyA gene encoding a phytase from the fungus Aspergillus fumigatus.
10p
xilank_of
22-07-2020
19
1
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