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Extracellular enzymatic activity
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In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4 )2 HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h.
14p
thiencuuchu
27-11-2021
17
1
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The growth of V. parahaemolyticus was varied among different salinity and shaking conditions. It showed the best growth at 2.0% NaCl and 240 rpm. No change in the enzymatic activity (EA) of the tested extracellular enzymes was observed while changing salinity except the significant decline of gelatinase from 3.49±0.19 to 2.77±0.17 mm following salinity increase (p < 0.05).
14p
spiritedaway36
25-11-2021
12
0
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Vibrio parahaemolyticusis a popular Gram-negative bacterium in the marine and estuarine regions. It can cause Early Mortality Syndrome (EMS), now named Acute Hepatopancreatic Necrosis Disease (AHPND), which resulted in severe losses to the shrimp culture. This study aimed to investigate the effect of pH, temperature, and oxygen-limited condition on the extracellular enzymatic activity of V. parahaemolyticus.
7p
trinhthamhodang9
04-12-2020
7
2
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Earlier we presented several lines of evidence that a 67-kDa laminin binding protein (LBP) inLeishmania donovani,that is different from the putative mammalian 67-kDa laminin receptor, may play an important role in the onset of leish-maniasis, as these parasites invade macrophages in various organs after migrating through the extracellular matrix. Here we describe the membrane orientation of thisLeish-manialaminin receptor.
8p
tumor12
20-04-2013
42
2
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A100-kDa proteinwith endoglucanase activitywas purified from Triton X-100 extract of cells of the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius. The enzyme exhibited activity towards carboxy methyl cel-lulose and oat spelt xylan with pH and temperature optima of 4 and 80C, respectively. Cloning and nucleotide sequence analysis of the corresponding gene (celB) revealed an ORF encoding a preprotein of 959 amino acids which is consistent with an extracellular localization.
10p
tumor12
20-04-2013
32
1
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The peptide sequence DSYG(893–896) of the sheep sodium pumpa1 subunit is highly conserved among all K + -trans-porting P-type ATPases. To obtain information about its function, single mutations were introduced and the mutants were expressed in yeast and analysed for enzymatic activity, ion recognition, and a/bsubunit interactions. Mutants of Ser894 or Tyr895 were all active. Conservative phenylalan-ine and tryptophan mutants of Tyr895 displayed properties that were similar to the properties of the wild-type enzyme....
11p
awards
05-04-2013
44
2
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