Freeze-thawing

The objective of study was to differentiate fresh from frozen-thawed chevon by evaluating citrate synthase enzymatic assay and the effects of repeated freeze– thaw cycles (0–2) on physico-chemical and microbiological parameters in longissimusdorsi of chevon.

9p nguaconbaynhay7 15-08-2020 16 1   Download

Semen cryopreservation allows crucial management of animal genetic diversity. However, the freezethaw process causes biochemical and physical alterations, impairing sperm energy-dependent functions. Currently, many chemicals are added to the media to enhance frozen-thawed sperm quality during artificial insemination.

11p viathena2711 08-10-2019 15 1   Download

To examine functions of two small heat shock proteins of Escherichia coli, IbpA and IbpB, we constructed His–IbpA and His–IbpB, in which a polyhistidine tag was fused to the N-terminals. Both purified His–IbpA and His–IbpB formed multimers, which have molecular masses of about 2.0– 3.0 MDa and consist of about 100–150 subunits.

11p system191 01-06-2013 35 2   Download

Two major approaches to cryopreservation are known, i.e., conventional freeze-thaw procedures and vitrification, which is defined as a glass-like solidification (Karlsson & Toner, 1996). While freeze-thaw procedures minimize the probability of intracellular ice formation, vitrification attempts to prevent ice formation throughout the entire sample during the cooling and warming process (Kuleshova et al., 2007). Recently, the potential of vitrification has been tested for tissue-engineered constructs.

364p kmkmkmkmkm 08-09-2012 71 7   Download