Gel electrophoresis

Ebook "Protein acetylation: Methods and protocols" expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction, and the biological response to protein acetylation.

279p dongmelo 27-05-2024 2 2   Download

Ebook "Detection and typing strategies for pathogenic Escherichia coli" will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years.

114p tudohanhtau1006 29-03-2024 4 1   Download

In ebook "Applications of MALDI-TOF spectroscopy" some topics involve the principles and the applications of ISD. Typically, MALDI is coupled with time-of-flight (TOF) analyzers with rather high resolution and a good cost performance, therefore MALDI-TOF-MS has become a nice combination in MS analysis.

220p coduathanh1122 27-03-2024 2 1   Download

Ebook "Proteomic methods in neuropsychiatric research (Advances in experimental medicine and biology, Volume 974)" will be of high interest to clinical scientists, physicians and pharmaceutical company scientists as it gives insights into the latest technologies enabling the revolution of personalized medicine. It is of direct interest to both technical and bench biomarker scientists as it gives step by step instructions on how to carry out each of the protocols.

362p duongthandue0501 28-02-2024 4 1   Download

Ebook "11th International conference on practical applications of computational biology & bioinformatics" research are increasingly driven by experimental techniques that challenge our ability to analyse, process and extract meaningful knowledge from the underlying data. The impressive capabilities of next-generation sequencing technologies, together with novel and constantly evolving, distinct types of omics data technologies, have created an increasingly complex set of challenges for the growing fields of Bioinformatics and Computational Biology.

345p lucchinguyen 28-12-2023 3 2   Download

Ebook "Hepatitis B virus: Methods and protocols" details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery.

301p lucchinguyen 28-12-2023 5 2   Download

Ebook "Difference gel electrophoresis: Methods and protocols" provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery.

310p lucchinguyen 28-12-2023 5 2   Download

Ebook "Bacterial protein secretion systems: Methods and protocols" details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport.

521p lucchinguyen 28-12-2023 4 2   Download

Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.

134p oursky10 04-12-2023 4 2   Download

Part 1 book "PCR mutation detection protocols" includes content: Conformation sensitive capillary electrophoresis, conformation sensitive gel electrophoresis, denaturing HPLC for mutation screening, in situ detection of human papillomavirus DNA after PCR-Amplification,.... and other contents.

153p oursky06 17-10-2023 1 1   Download

Salmonella enterica is one of the most dangerous food-borne pathogens posing a significant global concern especially to travelers returning from developing countries. The present study aimed to evaluate the phenotypic and genotypic antimicrobial resistances of Salmonella from chicken carcasses.

11p vimulcahy 18-09-2023 7 3   Download

Propofol is a safe and effective intravenous anesthetic that is widely used for the induction and maintenance of anesthesia during surgery. However, the mechanism by which propofol exerts its anesthetic effect remains unknown. The rapid onset of phosphorylation modifications coincides with that of propofol anesthesia.

10p viisaacnewton 26-04-2022 7 1   Download

During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis.

14p vidarwin 23-02-2022 9 1   Download

Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates.

8p vigandhi 23-02-2022 11 1   Download

Virus coat protein (CP) molecular weight was determined using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), giving a single band of 25 kDa within resolving gel. Immunocapture-reverse transcriptase-polymerase chain reaction

5p schindler 30-12-2021 5 0   Download

A new capillary electrophoresis method was developed for the simultaneous determination of dexpanthenol (DEX), lidocaine (LID), and mepyramine (MEP) in pharmaceutical preparations. The best results were obtained using 20 mM (pH 3.0) phosphate buffer as the background electrolyte. Separation was obtained using a fused-silica capillary (75 µm internal diameter, 50 cm total length, 41 cm effective length) and a potential of +30 kV at 20 ◦ C. Standards and samples were injected using a pressure injection at 50 mbar for 5 s and the analytes were monitored at a detection wavelength of 200 nm.

9p langthannam 29-12-2021 12 0   Download

Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP®R. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.

13p langthannam 29-12-2021 8 0   Download

In this study, some enzymology parameters and some antibiotics affecting the enzyme activity of 6- phosphogluconate dehydrogenase (6PGD) extracted from t the livers of fish from Lake Van (Chalcalburnus tarichi) were investigated because it is an important enzyme-producing NADPH, a reductive power, protecting the cell against the oxidative agents by producing reduced glutathione. The crude enzyme solution was obtained by using the affinity chromatography method.

9p langthannam 29-12-2021 11 0   Download

A series of novel positional oximes (2a–2d), O-methyl oxime ethers (3a–3d), and O-benzyl oxime ethers (4a–4d) were synthesized in high yields starting from their corresponding methyl 3-, 4-, 6-, and 13-keto tetradecanoates. The synthesized compounds were characterized by 1H NMR, 13C NMR, FT-IR, mass, and elemental analyses for their structures and (E) /(Z) isomerizations. Their DNA binding abilities were investigated in vitro by agarose gel electrophoresis. The antibacterial and antifungal activities were tested also in vitro against eleven bacterial strains and three fungal strains.

14p langthannam 29-12-2021 8 1   Download

In this study, a zinc(II) phthalocyanine compound (PcZn) substituted with octakis phenoxyacetamide was synthesized and characterized. Calf thymus DNA (CT-DNA) was used to determine the DNA binding properties of the zinc(II) phthalocyanine compound. This interaction of CT-DNA with PcZn was investigated using electronic absorption spectra, emission spectroscopy, a thermal melting temperature study, cyclic voltammetry, and viscosity measurements in a Tris-HCl buffer solution at a pH of 7.2.

11p tudichquannguyet 29-11-2021 5 0   Download