Genome walking

We describe a strategy for systematic amplification of chitin synthase genes (chs) in the filamentous ascomycetes plant-pathogenBotrytis cinereausing PCR with multiple degen-erate primers designed on specific and conserved sequence motifs. Eight distinctchsgeneswere isolated, namedBcchs I, II, IIIa, IIIb, IV, V, VIandVII. They probably constitute the entire chsmultigenic family of this fungus, as revealed by careful analysis of six euascomycetes genomes.

12p dell39 03-04-2013 37 4   Download

An NADH oxidase (NOX) was cloned from the genome ofThermococ-cus profundus (NOXtp) by genome walking, and the encoded protein was purified to homogeneity after expression in Escherichia coli.Subsequent analyses showed that it is an FAD-containing protein with a subunit molecular mass of 49 kDa that exists as a hexamer with a native molecular mass of 300 kDa.

11p vinaphone15 28-02-2013 39 3   Download

Genome walking is a molecular procedure for the direct identification of nucleotide sequences from purified genomes. The only requirement is the availability of a known nucleotide sequence from which to start.

25p cosis54 09-12-2012 55 2   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: A non-random walk through the genome...

6p thulanh19 09-11-2011 49 3   Download