Human telomeric

Telomerase is a specialized reverse transcriptase responsible for synthesizing telomeric DNA at the ends of chromosomes. Six subunits composing the telomerase complex have been cloned: hTR (human telomerase RNA), TEP1 (telomerase-associated protein 1), hTERT (human telomerase reverse transcriptase), hsp90 (heat shock protein 90), p23, and dyskerin. In this study, we investigated the role of each the telomerase subunit on the activity of telomerase.

9p research12 01-06-2013 28 3   Download

One of the most drastic post-translational modification of proteins in eu-karyotic cells is poly(ADP-ribosyl)ation, catalysed by a family enzymes termed poly(ADP-ribose) polymerases (PARPs). In the human genome, 18 different genes have been identified that all encode PARP family members.

14p fptmusic 11-04-2013 52 2   Download

The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K + is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogen-eity.

12p galaxyss3 19-03-2013 33 3   Download

This study examines the characteristics of binding of berberine to the human telomeric d[AG3(T2AG3 )3] quadruplex. By employing UV-visible spectroscopy, fluorescence spectroscopy and isothermal titration calorime-try, we found that the binding affinity of berberine to the human telomeric quadruplex is 10 6 .

13p galaxyss3 07-03-2013 35 3   Download

Naturally occurring G-rich DNA sequences that are able to form G-quad-ruplex structures appear as potential targets for anti-cancer chemotherapy, and therefore play an important role in cellular processes, such as cell aging, death and carcinogenesis.

9p vinaphone15 27-02-2013 45 3   Download

Eukaryotic pseudouridine synthases direct RNA pseudouridylation and bind H⁄ACA small nucleolar RNA (snoRNAs), which, in turn, may act as precursors of microRNA-like molecules. In humans, loss of pseudouridine synthase activity causes dyskeratosis congenita (DC), a complex systemic disorder characterized by cancer susceptibility, failures in ribosome biogen-esis and telomere stability, and defects in stem cell formation.

15p viettel02 19-02-2013 40 3   Download

The innate immune response of insects includes induced expression of genes encoding a variety of antimicrobial peptides. The signaling pathways that stimulate this gene expression have been well characterized by genetic analy-sis in Drosophila melanogaster, but are not well understood in most other insect species.

11p mobifone23 18-01-2013 48 4   Download

Telomeres play an important role in cellular aging and cancer. Human telomeric DNA and RNA G-rich sequences are capable of forming a four-stranded structure, known as the G-quadruplex. Such a structure might be important for telomere biology and a good target for drug design.

8p mobifone23 18-01-2013 42 6   Download

The 3¢-ends of human chromosomal DNA terminate in short single-stranded guanine-rich tandem-repeat sequences. In cancer cells, these are associated with the telomere-maintenance enzyme telomerase together with the end-binding protein hPOT1.

9p mobifone23 18-01-2013 41 5   Download

Thermodynamic and kinetic studies complement high-resolution structures of G-quadruplexes. Such studies are essential for a thorough understanding of the mechanisms that govern quadruplex folding and conformational changes in quadruplexes.

16p mobifone23 18-01-2013 37 5   Download