In vitro depleting

Ebook "Circular RNAs: Methods and protocols" provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

223p lucchinguyen 28-12-2023 3 2   Download

DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.

3p viaristotle 29-01-2022 10 0   Download

We further confirm the specificity of toxin-conjugated anti-CD137 mAb showing that only T cells that expressed CD137 underwent apoptosis by staining with toxin-FITC-mAb conjugates. We also found that toxinconjugated anti-CD137 mAb was able to delete allore-active T cells in vitro. Our result indicates that CD137 targeted delivery of toxin is a good strategy to delete antigen-specific T cells.

8p tamynhan8 04-11-2020 7 1   Download

Optimizing the safety and efficacy of standard chemotherapeutic agents such as cisplatin (CDDP) is of clinical relevance. Serum starvation in vitro and short-term food starvation in vivo both stress cells by the sudden depletion of paracrine growth stimulation.

10p vijisoo2711 30-09-2020 5 2   Download

Iron is essential for cell replication, metabolism and growth. Because neoplastic cells have high iron requirements due to their rapid proliferation, iron depletion may be a novel therapeutic strategy for cancer. Deferasirox (DFX), a novel oral iron chelator, has been successful in clinical trials in iron-overload patients and has been expected to become an anticancer agent.

11p vimale2711 25-08-2020 20 2   Download

Chromosome 7 open reading frame 24 (C7orf24) was originally identified as a highly expressed protein in various types of cancer, and later shown to be a γ-glutamylcyclotransferase (GGCT). GGCT depletion in cancer cells has anti-proliferative effects in vitro and in vivo, and it is therefore considered a promising candidate as a therapeutic target. However, the cellular events induced by GGCT depletion remain unclear.

9p vimale2711 25-08-2020 10 2   Download

Fetal bovine serum (FBS) contains a wide range of growth factors, hormones, vitamins, amino acids, fatty acids and trace elements required for cell growth. It was shown that animal sera contain also extracellular vesicles (EVs) with important biological properties; thus we wondered whether EVs present in FBS would influence muscle cell phenotype.

12p viuchiha2711 21-04-2020 10 1   Download

It has been considered that vitamin E is the major membrane bound antioxidant employed in humans, and thus this lipophilic chain breaking antioxidant has the ability to inhibit lipid peroxidation. Synergistic actions between vitamin C and vitamin E therefore appear important in their preventive activity against lipid peroxidation. Human trials and in-vitro studies have shown that oxidative stress causes a rapid depletion of vitamin C and vitamin E (reviewed by Bouayed & Bohn, 2010).

344p wqwqwqwqwq 23-07-2012 68 7   Download