In vitro Micropropagation protocol

The explant’s rhizognesis was carried out with a concentration of 0.5 mg / l ANA, before acclimatization on a substrate containing soil and sand in a ratio of 3:1. In conclusion, these protocols provide easy and cost-effective means to multiply cacti in vitro to preserve them in areas where their culture has been destroyed by cochineal.

7p mudbound 10-12-2021 14 0   Download

Thesis objectives: Evaluating the distribution of morphological characteristics, building a distribution map of the population of P. trimera and some species of the genus Paramignya; determining the genetic relationship of the P. trimera population by SSR markers; identification of DNA barcodes for identification of P. trimera; developed the protocol of in vitro micropropagation of P. trimera collected in Khanh Hoa, Vietnam.

27p beloveinhouse01 15-08-2021 19 3   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

In view of importance of Quince in the state economy and need for its rapid multiplication, the present investigation was laid to develop a commercial micropropagation protocol of Cydonia oblonga mill cv.SKAU-016.

9p caygaocaolon8 07-11-2020 14 2   Download

The present investigation was emphasized on development of an efficient in vitro micropropagation protocol for high frequency mass multiplication of true-to-type plants and to detect the genetic uniformity of micropropagated plants of Stevia using molecular markers.

13p nguathienthan8 20-10-2020 12 2   Download

The genetic fidelity of in vitro raised gerbera plants multiplied through micropropagation upto fifteen in vitro subcultures was assessed by using intersimple sequence repeat (ISSR) markers. 40 ISSR primers screened, 20 ISSR primers produced a total of 54 ISSRs clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (15) studied. Thus, a total 1094 bands were generated which exhibited homogeneous banding patterns with ISSR markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant.

7p quenchua6 15-06-2020 18 2   Download

Naga chilli or Bhut jolokia is extensively cultivated all over northeast India including Assam. This chilli is of high commercially value due to its pungent trait called capsaicin. The chilli is well known to have extreme potential for numerous pharmaceutical applications apart from being used for culinary purposes. Despite being such an important spice crop, demand of Naga chilli is deteriorating owing to natural cross pollination and mutation which in turn reduce its capsaicin content.

13p chauchaungayxua5 08-05-2020 10 0   Download

Litchi is a very important fruit of the Indian subcontinent and has a great demand in local and international market. But it is a very troublesome plant to cultivate and the current production rate cannot meet the growing demands of litchi. The in vitro techniques can be used to rapidly increase production. The present study was carried out to develop a rapid micropropagation protocol for litchi by studying the effect of medium and the explant used.

10p kethamoi4 18-04-2020 10 1   Download

The present experiment was conducted to standardize an efficient protocol for rapid multiplication of an important cultivar Kufri Lima. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutes followed by 0.1% HgCl2 treatment for 55 seconds was optimum for in vitro culture establishment and maximum survival percentage.

5p caygaocaolon4 04-04-2020 11 1   Download

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations.

5p caygaocaolon4 04-04-2020 12 1   Download

In this study culture mediums were tested to find a suitable commercially relevant production system for lily.

8p 12120609 18-03-2020 18 2   Download

Micropropagation is a highly sought after technique in the commercial production of orchid plants. This has the advantage of providing large number of plants in a short period of time. But a major constrain in the in vitro propagation technique is the somaclonal variation. It is important to produce true to type planting materials especially in case of the hybrids as they are more prone to variations. The true to type nature of the micropropagated plantlets can be confirmed by genetic fidelity analysis.

9p caygaocaolon3 27-02-2020 12 2   Download

Present investigation was carried out the effect of different photoperiod regimes on shoot bud induction, callus induction and shoot regeneration from callus culture in leaf explants of guggul. Standard protocols micropropagation protocol (1.5 mg/l BAP) for nodal segment and (2.0 mg/l Kn) for shoot apex explant and callus induction (2.0 mg/l 2,4-D) and regeneration protocol (1.5 mg/l Kn+ 1.0 mg/l 2,4-D)] were subjected to different photoperiod regimes (16:8, 14:10, 12:12 and 8:16). The cultures were incubated at 25±2°C with a light intensity of 3000 lux.

8p quenchua2 15-12-2019 19 0   Download

To meet the demands of good quality and true to type planting material of Vanda hybrid „Dr. Anek‟, in vitro micropropagation protocol was developed at Center for Plant Biotechnology and Molecular Biology (CPBMB), College of Horticulture, Kerala Agriculture University. The different explants tested to initiate the cultures were leaf, root, stem and inflorescence segments. The results of the experiment showed that treating the explants with 0.1 per cent carbendazim for 20 minutes, followed by 70 per cent ethanol for 5 minutes and 0.

12p quenchua2 15-12-2019 6 0   Download

Micropropagation of Indian Blackberry (Syzygium cumini L.) species locally called as Jamun, has always been a challenging task. In India, Jamun tree is an underutilized minor fruit crop species possessing a high medicinal and nutritional value. An efficient protocol for rapid shoot proliferation of Jamun (cv. Rajamun) from nodal segment of locally grown mature trees has been developed. Experiments were conducted at Biotech Centre, Dr. PDKV, Akola during 2016-18. The Lloyd and McCown woody plant medium (WPM) used as a basal media supplemented with growth regulators at varying concentrations.

6p kethamoi2 14-12-2019 13 0   Download

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.

9p cothumenhmong1 08-12-2019 18 0   Download

The present investigation entitled, “Studies on the effect of various sterilization procedures for in vitro propagation Of Carnation (Dianthus caryophyllus L.) was carried out at the Plant Tissue Culture Laboratory of Department of Floriculture and Landscape Architecture, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan (HP) as a refinement in already existing protocol to find suitable and less hazardous surface sterilization chemicals than Mercuric Chloride which is one of the most widely used surface sterilant in micropropagation of carnation.

5p cothumenhmong1 08-12-2019 15 1   Download

A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators.

5p viminotaur2711 30-10-2019 14 0   Download

This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA.

9p viartemis2711 22-10-2019 13 1   Download

The aim of the present study was to develop a simplified micropropagation protocol for this species, combining phases of in Vitro propagation and rooting and to assess the genetic stability of in Vitro regenerants using ISSR and flow cytometric analysis. The highest number of roots of all tested treatments was produced on MS medium supplemented with zeatin. Ten randomly chosen in Vitro regenerants were subjected to ISSR and flow cytometric analysis. Thus, the micropropagation method optimized here can be used for highly effective production of true-to-type plants of T.Leontopetaloides.

8p bichxuan01643027348 15-08-2019 22 1   Download