Isomerohydrolase

Generation of 11-cis-retinol from all-trans-retinyl ester in the retinal pigment epithelium is a critical step in the visual cycle and is essential for perception of light. Recent findings from cell culture models suggest that protein RPE65 is the retinoid isomerohydrolase that catalyzes the reaction. However, previous attempts to detect the enzymatic activity of purified RPE65 were unsuccessful, and thus its enzymatic function remains contro-versial.

11p viettel02 22-02-2013 22 2   Download

Cone photoreceptors have faster light responses than rods and a higher demand for 11-cisretinal (11cRAL), the chromophore of visual pigments. RPE65 is the isomerohydrolase in the retinal pigment epithelium (RPE) that converts all-transretinyl ester to 11-cisretinol, a key step in the visual cycle for regenerating 11cRAL.

14p cosis54 04-01-2013 29 2   Download