Making PCR

Part 1 book "PCR methods in foods" includes content: PCR basics, the mythology of PCR - A warning to the wise, sample preparation for PCR, making PCR a normal routine of the food microbiology lab.

73p oursky06 17-10-2023 5 1   Download

Nucleic acid amplification technologies (NAAT) are advancing our ability to make rapid molecular diagnoses in patients with serious culture negative infections. This is the first report of PCR coupled to electrospray ionization mass spectrometry use in the evaluation of complicated community acquired pneumonia in a pediatric patient.

5p viisaacnewton 26-04-2022 17 2   Download

Reverse transcription quantitative PCR (RT-qPCR) is widely used for gene expression analysis in various organisms. Its accuracy largely relies on the stability of reference genes, making reference gene selection a vital step in RT-qPCR experiments. However, previous studies in mollusks only focused on the reference genes widely used in vertebrates.

12p visilicon2711 20-08-2021 8 1   Download

Green gram is a widely cultivated pulse crop rich in protein, essential amino acids and vitamin-B. The quality and quantity of DNA are very important for amplification by PCR. Although quantity of DNA required per reaction in PCR is very low, quality is very crucial. Also, to carry out large number of PCR reactions for genotyping, a good amount of DNA is required. Presence of contaminants like phenols makes it difficult to get good quality DNA from mungbean.

9p caygaocaolon8 07-11-2020 15 2   Download

Assessing library diversity is an important control step in a directed evolution experiment. To do this, a limited amount of colonies from a test library are sequenced and tested. In the case of an error-prone PCR library, the spectrum of the identified mutations — the proportions of mutations of a specific nucleobase to another— is calculated enabling the user to make more informed predictions on library diversity and coverage.

5p vioklahoma2711 19-11-2020 12 2   Download

The JAK2 V617F mutation is the most frequent somatic change in myeloproliferative neoplasms, making it an important tumour-specific marker for diagnostic purposes and for the detection of minimal residual disease.

13p vijennie2711 29-09-2020 15 1   Download

The mulberry silkworm, Bombyx mori L is a Lepidopteran insect, life cycle of which include: Egg, Larval Instars, Pupa within silky cocoon and adult moth. It is purely domesticated insect since long, which make it a quite delicate venture, easily susceptible to viral and other diseases. The viral diseases are difficult to manage due to a very short life cycle of silkworm. One of the most effective solutions is a timely detection of such infection so that to stop spread of the disease.

11p chauchaungayxua6 26-06-2020 14 1   Download

A brief study was carried on forty Staphylococcus aureus isolates from Bovine milk from Ludhiana, Punjab, India for detecting the antibiotic resistance profile using E-strips and genotypically targeting various antibiotic resistance genes. The antibiotics used for phenotypic resistance detection were Penicillin, Gentamicin, Erythromycin, Clindamycin, Tetracycline, Chloramphenicol, Ciprofloxacin, Trimethoprim-sulphamethoxazole, Oxacillin, Vancomycin and Amoxyclav.

14p nguaconbaynhay5 16-05-2020 6 0   Download

Targeting Induced Local Lesions in Genome (TILLING) is a general reverse technique that combines chemical mutagenesis with PCR based screening to identity point mutations in regions of interest. The strategy works with a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms in gene of interest. The main principles of TILLING include making EMS-mutagenized population, targeting gene of interest with PCR primers and searching rare mutants among many individuals.

7p chauchaungayxua5 05-05-2020 14 2   Download

Far infrared radiation (FIR) has been widely used to treat chronic diseases and symptoms; however, the underlying mechanism remains unclear. As gut microbiota (GM) markedly impact the host’s physiology, making GM a potential target for the therapeutic evaluation of FIR. C57BL/6J mice were exposed to five times of 2 min-FIR exposure on the abdomen, with a two-hour interval of each exposure within one day. Fecal samples were collected on day one and day 25 after the FIR/control treatment, and the extracted fecal DNAs were evaluated using ERIC-PCR and 16S amplicon sequencing.

8p partimesinhvien 08-05-2020 6 0   Download

Dermatophytes are a group of closely related keratinophilic fungi that can invade keratinized humans and animals tissues such as skin, hair and nails causing dermatophytosis. They are an important cause of superficial fungal infection. Conventional methods like potassium hydroxide (KOH) direct microscopy and fungal culture lacks the ability to make an early and specific diagnosis, as it takes about four weeks for the culture to grow on Sabourauds dextrose agar.

5p kethamoi4 18-04-2020 17 1   Download

The study was aimed to detect virulence associated genes of Salmonella serovars, which were isolated from raw pork samples of unorganised butcher shops of Aizawl and Imphal. A total of 5 Salmonella isolates (Aizawl=2, Imphal=3), belonging to Salmonella vircho (n=4), and Salmonella typhimurium (n=1), were obtained from 200 samples (100= Aizawl and 100= Imphal) and screened for five virulence associated genes, namely invA, stn, pefA, sefC, spvC by Polymerase Chain Reaction (PCR) technique.

9p trinhthamhodang3 12-02-2020 13 1   Download

PCR detection of blaIMP gene in Imipenem resistant E. coli is the aim ot this study. Total of n=66 of n=23 Imipenem resistant E. coli were selected for the present study based on the drug resistance pattern and their MIC to Imipenem. Phenotypic detection of MBL production was carried out and subsequently, PCR amplification of blaIMP was carried out. Among selected (n=23) Imipenem resistant E. coli (IREC), 100% (n=23) strains were positive for Imipenem mediated MBL production. Twenty-three MBL positive IREC isolates were shown presence of Plasmid DNA, with size measured about approx. 6kb.

6p cothumenhmong1 11-12-2019 11 0   Download

Efficiencies of local bacterial isolates in malathion degradation were investigated. Five bacterial isolates obtained from agricultural waste water were selected due to their ability to grow in minimal salt media, supplied with 250 ppm malathion as sole source of carbon and phosphorus. The purified bacterial isolates (MOS-1, MOS-2, MOS-3, MOS-4 and MOS-5) were characterised and identified using a combination of cellular profile (SDS-PAGE), genetic make up profile (RAPD-PCR), and morphological and biochemical characteristics.

5p kethamoi1 20-11-2019 12 0   Download

Streptococcus suis serotype 2 (SS2) is one of the most important pathogens in the porcine industry and an important zoonotic agent. The absence of suitable vaccine or virulence markers makes SS2 infections more difficult to control. An immunoproteomics approach is used for identifying antigenic proteins in SS2 recognized enolase, which may represent strainspecific antigenic proteins and potential protective antigens. This study aims to clone, express enolase gene from SS2 and use western blotting to evaluate the antigenicity.

6p caygaocaolon1 13-11-2019 22 1   Download

Bacterial leaf blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a destructive disease in rice fields. Can Tho is one of the most important rice-growing areas in the Mekong Delta, which is vulnerable to climate change, making the disease more damaging in this region. Deployment of resistance genes is considered an economic and eco-friendly approach to control the disease. However, Xoo exists in different races with diverse reactions on different resistance genes.

9p viathena2711 08-10-2019 17 0   Download

Borrelia burgdorferi sensu stricto is the predominant cause of Lyme disease. B. burgdorferi strains carry several linear and circular plasmids and this may be also used as a distinguishing property of the bacteria. The presence of a mixed population of linear and circular plasmids makes separation and characterization of each kind very difficult.

8p vitamix 15-02-2019 24 1   Download

There is an urgent need for developing crops with greater tolerance to environmental stresses. This is even more important for fiber crops, which are being pushed to the marginal low-productive lands in order to make more room for food crops.

6p vivant2711 01-02-2019 23 1   Download

The sensitivity of Polymerase Chain Reaction (PCR) makes it a potential diagnostic test for detection of M. tuberculosis in samples with low bacillary load. Aim: To assess the efficiency of PCR as compared to routine diagnostics in detection of M. tuberculosis from sputum samples of suspects referred to a tuberculosis clinic and those identified during a morbidity survey. Methods: Respiratory samples (sputum with or without saliva) from 144 individuals were examined by PCR, using MPB64 primers, culture and microscopy.

8p taisaocothedung 12-01-2013 64 2   Download

More discrete sequence alterations rely heavily on the use of the PCR, which allows rapid gene amplification and analysis. Moreover, PCR makes it possible to perform genetic testing and mutational analysis with small amounts of DNA extracted from leukocytes or even from single cells, buccal cells, or hair roots. Screening for point mutations can be performed by numerous methods (Table 62-9); most are based on the recognition of mismatches between nucleic acid duplexes, electrophoretic separation of single- or double-stranded DNA, or sequencing of DNA fragments amplified by PCR.

10p konheokonmummim 03-12-2010 64 2   Download