Microbial gene identifiers
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This shows an intraspecies difference between Bacteroides species in the Philippines and Japan. This difference is a result of variations in the gut microbiome such as host species, diet, and geography. Results of this study will expand the present sequence database of Bacteroides from ducks that can be used in identifying sources of fecal pollution in the environment.
7p mudbound 10-12-2021 9 0 Download
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In shotgun metagenomics, microbial communities are studied through direct sequencing of DNA without any prior cultivation. By comparing gene abundances estimated from the generated sequencing reads, functional differences between the communities can be identified. However, gene abundance data is affected by high levels of systematic variability, which can greatly reduce the statistical power and introduce false positives. Normalization, which is the process where systematic variability is identified and removed, is therefore a vital part of the data analysis.
17p vibeauty 23-10-2021 10 2 Download
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In accordance with their immense catabolic diversity, Rhodococcus spp. possess large and complex genomes, which contain a multiplicity of catabolic genes, a high genetic redundancy of biosynthetic pathways and a sophisticated regulatory network. The present study aimed to identify genes involved in the o-xylene degradation in R. opacus strain R7 through a genome-based approach.
17p vitzuyu2711 29-09-2021 9 1 Download
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This study reports the analyses of the rhizospheric microbiome of the groundnut (Arachis hypogaea L.) from Gujarat, India. Samples were collected using standard protocols and 16S rRNA gene V3–V4 region amplicon sequencing was performed to identify the microbial communities prevalent in the rhizosphere.
9p cothumenhmong11 11-05-2021 9 2 Download
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Cross-species comparisons of gene neighborhoods (also called genomic contexts) in microbes may provide insight into determining functionally related or co-regulated sets of genes, suggest annotations of previously un-annotated genes, and help to identify horizontal gene transfer events across microbial species.
8p viwyoming2711 16-12-2020 16 1 Download
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The scientific literature contains millions of microbial gene identifiers within the full text and tables, but these annotations rarely get incorporated into public sequence databases. We propose to utilize the Open Access (OA) subset of PubMed Central (PMC) as a gene annotation database and have developed an R package called pmcXML to automatically mine and extract locus tags from full text, tables and supplements.
8p vikentucky2711 26-11-2020 15 0 Download
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Genomic islands play an important role in microbial genome evolution, providing a mechanism for strains to adapt to new ecological conditions. A variety of computational methods, both genome-composition based and comparative, have been developed to identify them.
11p viconnecticut2711 28-10-2020 14 2 Download
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Detection of Methicillin resistant strains of Staphylococcus aureus using phenotypic and genotypic methods in a tertiary care hospital Institution: Institute of Microbiology, Madras Medical College. Abstract: Purpose: Methicillin resistant S. aureus (MRSA) are significant pathogens that have emerged over the past 30 years to cause both nosocomial and community acquired infections. This study has been undertaken to compare the conventional and molecular methods in detecting MRSA. S. aureus was isolated from pus samples and identified by Gram stain and Catalase test.
7p nguaconbaynhay8 13-10-2020 16 2 Download
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Listeria monocytogenes is widely distributed and found in many food commodities. Post processing contamination is the major source and cross contamination may also occur at the retail shop and also in products due to improper hygienic practices. A study was conducted to identify the presence of L. monocytogenes in chicken meat by polymerase chain reaction. 40 samples were collected from different areas of Chennai and were tested for the presence of L. monocytogenes by targeting prfA gene with 290bp by PCR. None of the samples were shown to be positive for L. monocytogenes.
4p angicungduoc4 26-04-2020 10 0 Download
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The rapid emergence of multiresistant microbial pathogens is a serious threat to human health. Extended spectrum beta lactamase (ESBL)-producing Escherichia coli is a superbug causing worldwide outbreaks, CTX-M has become the most commonly detected ESBL genotype; these enzymes have become predominant worldwide, the most common type of CTX-M reported in various geographic regions is CTX-M15. A total of 80 Escherichia coli (E. coli) isolates were recovered from different clinical samples of intensive care units (ICUs) patients.
13p nguathienthan2 26-12-2019 17 1 Download
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Rice genome harbours many resistance genes (R-genes) with tremendous allelic diversity, constituting a robust immune system effective against microbial pathogens like rice blast fungus M. oryzae. Nevertheless, few functional R-genes have been identified for rice blast resistance. Wild species of cultivated plants are treasure trove for important agronomic traits. The wild rice Oryza rufipogon is resistant to many virulent strains of Magnaporthe oryzae.
9p chauchaungayxua1 04-12-2019 14 1 Download
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A plant’s microbiota has various implications for the plant’s health and performance; however, the roles of many microbial lineages, particularly Archaea, have not been explored in detail. In the present study, analysis of archaea-specific 16S rRNA gene fragments and shotgun-sequenced metagenomes was combined with visualization techniques to obtain the first insights into the archaeome of a common salad plant, arugula (Eruca sativa Mill.).
10p caygaocaolon1 13-11-2019 10 0 Download
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The Eurasian spruce bark beetle, Ips typographus L. (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis.
12p vivant2711 01-02-2019 34 2 Download
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The serotype a-specific polysaccharide antigen ofActinoba-cillus actinomycetemcomitansis an unusual sugar, 6-deoxy-D-talose.Guanosinediphosphate (GDP)-6-deoxy-D-talose is the activated sugar nucleotide form of 6-deoxy-D-talose, which has been identified as a constituent of only a few microbial polysaccharides. In this paper, we identify two genes encoding GDP-6-deoxy-D-talose synthetic enzymes, GDP-a-D-mannose 4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose reductase, in the gene cluster required for the biosynthesis of serotype a-specific polysaccharide anti-gen from A.
9p tumor12 22-04-2013 29 1 Download