Modifying specific DNA sequence

Nuclease-based technologies have been developed that enable targeting of specific DNA sequences directly in the zygote. These approaches provide an opportunity to modify the genomes of inbred mice, and allow the removal of strain-specific mutations that confound phenotypic assessment.

12p vioraclene 31-03-2024 4 2   Download

Traditionally, generation of new plants with improved or desirable features has relied on laborious and timeconsuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes.

12p vijichea2711 28-05-2021 11 1   Download

Genome editing is a technique in which changes are made in the DNA of any organism. A nuclease promotes breaks in the DNA at a specific sequence which is repaired by several mechanisms. It is one of the efficient technologies which enable us to change and edit genome of any organism precisely and accurately. Multiple genome editing technologies have been employed, including zinc finger nucleases, mega nucleases, clustered regularly interspaced short palindromic repeats (CRISPR) along with Cas9 protein and transcription activator-like effector nucleases to modify genome of an organism.

9p trinhthamhodang1218 26-02-2021 13 3   Download

Since their first commercialization, the diversity of taxa and the genetic composition of transgene sequences in genetically modified plants (GMOs) are constantly increasing. To date, the detection of GMOs and derived products is commonly performed by PCR-based methods targeting specific DNA sequences introduced into the host genome.

14p viwyoming2711 16-12-2020 10 0   Download

GmrSD is a modification-dependent restriction endonuclease that specifically targets and cleaves glucosylated hydroxymethylcytosine (glc-HMC) modified DNA. It is encoded either as two separate single-domain GmrS and GmrD proteins or as a single protein carrying both domains. Previous studies suggested that GmrS acts as endonuclease and NTPase whereas GmrD binds DNA.

16p vioklahoma2711 19-11-2020 9 0   Download

Movement protein is a non-structural protein encoded by plant viruses to allow their movement from infected cell to neighbouring one. It defined as plant viruses- encoded factors that interact with plasmodesmata to mediate the intracellular spread of virus infection. viruses subvert an intracellular communication network that supports the trafficking of wide spectrum of endogenous protein and ribonucleo protein complexes that play non-cell autonomous roles in development and physiological processes.

17p angicungduoc6 22-07-2020 17 3   Download