Molecular cloning methods

Chapter 4 review the fundamentals of gene structure and function, we are ready to start a more detailed study of molecular biology. The main focus of chapter will be the experiments that molecular biologists have performed to elucidate the structure and function of genes.

67p tangtuy05 30-03-2016 93 6   Download

Until now, only a small amount of information is available about tomato allergens. In the present study, a glycosylated allergen of tomato (Lycopersicon esculentum), Lyc e 2, was purified from tomato extract by a two-step FPLC method. The cDNA of two different isoforms of the protein, Lyc e 2.01 and Lyc e 2.02, was cloned into the bacterial expressionvector pET100D. The recombinant proteinswere purified by electroelution and refolded.

11p tumor12 20-04-2013 32 3   Download