Molecular detection of Brucella spp

The present study was carried out for the isolation, identification and molecular characterization of Brucella species. A total of 50 samples were collected from cattle and buffalo suffering from abortions and other reproductive disorders in and around Ludhiana, Punjab. Out of the 50 samples of fetal stomach contents (25), uterine discharges (10), vaginal mucus (8) and placenta (7) processed for isolation of Brucella of which a total of four isolate were obtained and identified biochemically. All the 4 isolates were typed as biotype 1.

5p trinhthamhodang1213 29-05-2020 6 0   Download

Brucellosis is a worldwide re-emerging zoonotic disease of public health and economic importance. It affects a large number of domestic as well as wild animals and results in heavy losses to the animal husbandry sector. The direct culture of bacteria and serological test are the gold standard for Brucella spp. identification in the clinical samples. However, these assays have various limitations therefore PCR can be a potential tool to address aforesaid limitations and can be used for early detection of causative agents in disease condition.

16p cothumenhmong1 11-12-2019 9 1   Download

Brucellosis is one of the world’s major zoonoses that affect wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis, the disease is still endemic among cattle, buffaloes, sheep and goats. In many developing countries including India, Brucella melitensis is of much concern due to heavy economic losses to animal husbandry sector as well as humans. The present study was carried out to detect and characterize B.

7p nguaconbaynhay1 04-12-2019 16 0   Download