Multi-wavelength analysis
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Starting with oligopeptide sequences and using PCR, the gene of the cyclodextrinase fromFlavobacteriumsp. no. 92 wasderivedfrom thegenomicDNA.Thegenewas sequenced and expressed inEscherichia coli; the gene pro-duct was purified and crystallized. An X-ray diffraction analysis using seleno-methionines with multiwavelength anomalous diffraction techniques yielded the refined 3D structure at 2.1 A ˚ resolution.
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