Myristoylation

In rod phototransduction, cyclic GMP synthesis by mem-brane bound guanylate cyclase ROS-GC1 is under Ca 2+ -dependent negative feedback control mediated by guanylate cyclase-activating proteins, GCAP-1 and GCAP-2. The cellular concentration of GCAP-1 and GCAP-2 approxi-mately sums to the cellular concentration of a functional ROS-GC1 dimer. Both GCAPs increase the catalytic effi-ciency (kcat /Km) of ROS-GC1. However, the presence of a myristoyl group in GCAP-1 has a strong impact on the regulation of ROS-GC1, this is in contrast to GCAP-2....

8p tumor12 20-04-2013 38 2   Download

HR12 is anovel farnesyltransferase inhibitor (FTI).Wehave shown previously that HR12 induces phenotypic reversion of H-ras V12 -transformed Rat1 (Rat1/ras) fibroblasts. This reversion was characterized by formation of cell–cell con-tacts, focal adhesions and stress fibers. Here we show that HR12 inhibits anchorage independent and dependent growth of Rat1/ras cells. HR12 also suppresses motility and proliferation of Rat1/ras cells, in a wound healing assay. Rat1 fibroblasts transformed with myristoylated H-ras V12 (Rat1/myr-ras) were resistant to HR12....

14p fptmusic 16-04-2013 38 2   Download

The N-terminally myristoylated, 19-amino acid peptide, corresponding to the amino terminus of the neuronal protein NAP-22 (NAP-22 peptide) is a naturally occurring peptide that had been shown by fluorescence to cause the sequestering of a Bodipy-labeled PtdIns(4,5)P2 in a cholesterol-depend-ent manner. The present work, using differential scanning calorimetry (DSC), extends the observation that formation of a PtdIns(4,5)P2-rich domain is cholesterol dependent and shows that it also leads to the forma-tion of a cholesterol-depleted domain. ...

12p awards 06-04-2013 28 2   Download

In order to determine the amino-terminal sequence requirements for protein N-myristoylation, site-directed mutagenesis of the N-terminal region was performed using tumor necrosis factor (TNF) mutants as model substrate proteins. Subsequently, the susceptibility of thesemutants to protein N-myristoylation was evaluated by metabolic labe-ling in anin vitrotranslation systemusing rabbit reticulocyte lysate. A TNF mutant having the sequence MGAAAAA AAA at its N-terminus was used as the starting sequence to identify elements critical for protein N-myristoylation....

12p dell39 03-04-2013 43 4   Download

A hydrophilic cation-binding protein, PCaP1, was found to be stably bound to the plasma membrane inArabidopsis thaliana. PCaP1 was quanti-fied to account for 0.03–0.08% of the crude membrane fractions from roots and shoots.

16p media19 06-03-2013 39 3   Download

Protein N-myristoylation plays key roles in various cellular functions in eukaryotic organisms. To clarify the relationship between the efficiency of protein N-myristoylation and the amino acid sequence of the substrate in plants, we have applied a wheat germ cell-free translation system with high protein productivity to examine the N-myristoylation of various wild-type and mutant forms ofArabidopsis thalianaproteins.

12p viettel02 19-02-2013 76 4   Download

Activation of the c-JUN N-terminal kinase (JNK) pathway is implicated in a number of important physiological processes, from embryonic morpho-genesis to cell survival and apoptosis. JNK stimulatory phosphatase 1 (JSP1) is a member of the dual-specificity phosphatase subfamily of protein tyrosine phosphatases.

11p mobifone23 21-01-2013 48 3   Download