Nodal segments

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations.

5p caygaocaolon4 04-04-2020 12 1   Download

This study is to investigate the in vitro propagation and ex vitro root formation of Gnetum gnemon var. griffithii Markgr (G. gnemon), one of the nutrient-rich forest vegetables. The results showed that MS medium supplemented with 1.5 mg l-1 BAP was the most suitable combination for shoot formation after 90 days of culture. The combination between 1 mg l-1 BAP and 0.5 mg l-1 NAA gave the best in vitro rooting rate (40%) after 60 days culture.

9p shiwo_ding7 05-06-2019 16 1   Download