Nucleoside phosphorylase

Substrate properties of xanthine (Xan) and xanthosine (Xao) for purine nucleoside phosphorylases (PNP) of mammalian origin have been reported previously, but only at a single arbitrarily selected pH and with no kinetic con-stants. Additionally, studies have not taken into account the fact that, at physiological pH, Xao (pKa¼5.7) is a mono-anion, while Xan (pKa¼7.7) is an equilibrium mixture of the neutral and monoanionic forms.

10p research12 23-04-2013 41 2   Download

We report here the characterization of the first mammalian-like purine nucleoside phosphorylase from the hyperthermophilic archaeonPyrococcus furiosus (PfPNP). The gene PF0853 encoding PfPNP was cloned and expressed in Escherichia coliand the recombinant protein was purified to homogeneity.

14p galaxyss3 21-03-2013 44 4   Download

5¢-Deoxy-5¢-methylthioadenosine phosphorylase II fromSulfolobus solfatari-cus (SsMTAPII) and purine nucleoside phosphorylase from Pyrococcus furiosus (PfPNP) are hyperthermophilic purine nucleoside phosphorylases stabilized by intrasubunit disulfide bonds. In their C-terminus, both enzymes harbour a CXC motif analogous to the CXXC motif present at the active site of eukaryotic protein disulfide isomerase.

7p viettel02 20-02-2013 37 3   Download

Genetic deficiency of purine nucleoside phosphorylase (PNP; EC 2.4.2.1) activity leads to a severe selective disorder of T-cell function. Therefore, potent inhibitors of mammalian PNP are expected to act as selective immunosuppressive agents against, for example, T-cell cancers and some autoimmune diseases.

14p mobifone23 18-01-2013 54 5   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Pig gene mapping : assignment of the genes for mannosephosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no

10p toshiba21 16-11-2011 46 3   Download