Nucleotide levels

WhenSaccharomyces cerevisiaecells, grown in galactose, glucose or mannose, were treated with 1.5 mMhydrogen peroxide (H2O2) for 30 min, an important decrease in the ATP, and a less extensive decrease in the GTP, CTP, UTP and ADP-ribose levels was estimated. Concomitantly a net increase in the inosine levels was observed. Treatment with 83mMmenadionepromoted theappearanceof acompound similar to adenosine but no appreciable changes in the nucleotide content of yeast cells, grown either in glucose or galactose....

12p tumor12 20-04-2013 42 3   Download

Volkensin, a type 2 ribosome-inactivating protein from the roots of Adenia volkensiiHarms (kilyambiti plant) was characterized both at the protein and nucleotide level by direct amino acid sequencing and cloning of the gene enco-ding the protein. Gene sequence analysis revealed that vol-kensin is encoded by a 1569-bp ORF (523 amino acid residues) without introns, withan internal linker sequence of 45 bp.

10p dell39 03-04-2013 69 4   Download

The activity of NTPDase (EC 3.6.1.5, apyrase, CD39) was verified in plate-lets from patients with increasing cholesterol levels. A possible association between cholesterol levels and inflammatory markers, such as oxidized low-density lipoprotein, highly sensitive C-reactive protein and oxidized low-density lipoprotein autoantibodies, was also investigated.

8p galaxyss3 19-03-2013 47 4   Download

This study characterized the human apolipoprotein H [APOH; b2-glycopro-tein I (b2GPI)] promoter and its variants byin vitro functional experiments and investigated their relationship with human plasmab2GPI levels. We examined the individual effects of 12 APOHpromoter single nucleotide polymorphisms in the 5¢ flanking region ofAPOH(1.4 kb) on luciferase activity in COS-1 cells and HepG2 cells and their impact on plasma...

13p mobifone23 18-01-2013 48 3   Download

Cyclophilin D was recently shown to bind to and decrease the activity of F0F1 -ATP synthase in submitochondrial particles and permeabilized mito-chondria [Giorgio Vet al. (2009) J Biol Chem, 284, 33982–33988]. Cyclo-philin D binding decreased both ATP synthesis and hydrolysis rates.

14p cosis54 05-01-2013 31 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L.) genes involved in frost response

14p panasonic02 14-12-2011 54 5   Download