Oligonucleotide design

Part 1 book "PCR mutation detection protocols" includes content: Conformation sensitive capillary electrophoresis, conformation sensitive gel electrophoresis, denaturing HPLC for mutation screening, in situ detection of human papillomavirus DNA after PCR-Amplification,.... and other contents.

153p oursky06 17-10-2023 1 1   Download

DNA microarrays have become ubiquitous in biological and medical research. The most difficult problem that needs to be solved is the design of DNA oligonucleotides that (i) are highly specific, that is, bind only to the intended target, (ii) cover the highest possible number of genes, that is, all genes that allow such unique regions, and (iii) are computed fast. None of the existing programs meet all these criteria.

8p viwyoming2711 16-12-2020 14 1   Download

A clear difference in the enthalpy changes derived from spectroscopic and calorimetric measurements has recently been shown. The exact interpretation of this deviation varied from study to study, but it was generally attributed to the non-two-state transition and heat capacity change. Although the temperature-dependent thermodynamics of the duplex formation was often implied, systemic and extensive studies have been lacking in universally assigning the appropriate thermodynamic parameter sets.

10p system191 01-06-2013 34 3   Download

Special oligonucleotides for targeted gene correction have attracted increasing attention recently, one of which is the chimericRNAÆDNAoligonucleotide (RDO) system. RDOs for targeted gene correction were first designed in 1996, and are typically 68 nucleotides in length including continuous RNA andDNA sequences (RNA is 2¢-O-methyl-modified).

6p tumor12 22-04-2013 31 1   Download

Embryos and larvae of the brine shrimp,Artemia francis-cana, were shown previously to possess a protein, now termed p49, which cross-links microtubules in vitro. Molecular characteristics of p49 were described, but the protein’s identity and its role in the cell were not determined. Degenerate oligonucleotide primers designed on the basis of peptide sequence obtained by Edman degradation during this studywereusedtogeneratep49cDNAsbyRT-PCRand thesewere clonedand sequenced.

11p fptmusic 12-04-2013 50 2   Download