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Optimized codons
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Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli).
10p
vimulcahy
18-09-2023
4
3
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In a previous study, a gene GL0694641 coding for endoglucanase containing 3 domains GH5- CBM72-CBM72 was exploited from metagenomic DNA data of bacteria in Vietnamese goats’ rumen. The gene (eg5) encoding the mature enzyme (without signal peptide coding sequence) was optimized codons, artificially synthesized, and inserted into the pET22b(+) vector at NcoI and XhoI to generate expression vector pET22-eg5 for expression of the gene in Eschrichia coli.
11p
vicaptainmarvel
21-04-2023
5
2
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Most amino acids are encoded by multiple synonymous codons. However, synonymous codons are not used equally, and this biased codon use varies between different organisms. It has previously been shown that both selection acting to increase codon translational efficiency and selection acting to decrease codon biosynthetic cost contribute to differences in codon bias.
11p
vigalileogalilei
27-02-2022
19
1
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We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We apply our method to identify six single nucleotide mutations that recover 59% of the fitness defect exhibited by the 63-codon E. coli strain C321.ΔA.
12p
vialfrednobel
29-01-2022
11
0
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Synonymous mutations do not change the protein sequences. Automatically, they have been regarded as neutral events and are ignored in the mutation-based cancer studies. However, synonymous mutations will change the codon optimality, resulting in altered translational velocity.
13p
vianrose2711
27-04-2021
9
2
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In order to optimize protein production in expression systems, analysis of codon usage patterns is very important. In this regard, chloroplast has special importance due to its small size and high copy number of genome. Vitis vinifera is the most important fruit species in the modern world. In this research, the complete nucleotide sequence of the chloroplast genome in Vitis vinifera was studied, and then analyzed using CodonW software.
7p
chauchaungayxua11
23-03-2021
12
2
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Codon usage plays a crucial role when recombinant proteins are expressed in different organisms. This is especially the case if the codon usage frequency of the organism of origin and the target host organism differ significantly, for example when a human gene is expressed in E. coli.
6p
vikentucky2711
24-11-2020
14
1
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Since the recombinant protein was discovered, it has become more popular in many aspects of life science. The value of global pharmaceutical market was $87 billion in 2008 and the sales for industrial enzyme exceeded $4 billion in 2012. This is strong evidence showing the great potential of recombinant protein.
10p
vioklahoma2711
19-11-2020
13
2
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Due to the degeneracy of the genetic code, most amino acids can be encoded by multiple synonymous codons. Synonymous codons naturally occur with different frequencies in different organisms. The choice of codons may affect protein expression, structure, and function.
10p
viflorida2711
30-10-2020
13
2
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In our previous study, a novel liver-targeting fusion interferon (IFN-CSP) combining IFN α2b with plasmodium region I peptide was successfully constructed. IFN-CSP has significant inhibition effects on HBV-DNA replication in HepG2.2.15 cells.
12p
viuchiha2711
21-04-2020
13
1
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Neurotrophic factors influence survival, differentiation, proliferation and death of neuronal cells within the central nervous system. Human ciliary neurotrophic factor (hCNTF) has neuroprotective properties and is also known to influence energy balance.
11p
viuchiha2711
21-04-2020
8
0
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Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptomyces TGase, TGase is usually expressed in an inactive pro-TGase form, which is then converted to active TGase by the addition of activating proteases in vitro.
9p
vihamax2711
21-04-2020
7
0
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Cyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-Dglucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical industries.
10p
vihamax2711
21-04-2020
14
1
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Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical methods.
12p
vihamax2711
21-04-2020
10
0
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Defensins are cationic antimicrobial peptides with a broad range of activities against bacteria and fungi. In the present study, the entire coding sequence of codon-optimized Bovine Neutrophil b-Defensin 2 (BNBD2) was designed and placed upstream of Trx coding sequence into the pET-48b (+) vector. Furthermore, the codon-optimized pelB signal sequences were also added to the upstream of BNBD2 for periplasmic localization. The periplasmic sorting of recombinant b-Defensin 2 was evaluated by osmotic shock and SDS–PAGE on the released proteins.
7p
kequaidan1
16-11-2019
17
0
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In the present study, a novel pre-miniproinsulin analogue was designed to have a short 9 residue sequence replacing the 35 residue C-chain, one lysine and one arginine added to the C-terminus of the B-chain in combination with glycine and arginine substitution at A21 and B29, respectively, and a 16-residue fusion partner comprising the pentapeptide sequence (PSDKP) of the N-terminus of human tumor necrosis factor-a (TNF-a), 6 histidine residues for Ni2+ chelated affinity purification and a pentapeptide ending with methionine for ease of chemical cleavage fused at the N-terminus.
9p
trinhthamhodang1
16-11-2019
23
1
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Interleukin-6 (IL-6) là một cytokine đa chức năng có hoạt tính rộng và có tác động lên hầu hết các loại tế bào của hệ miễn dịch như tế bào B, tế bào T, tế bào gan, tiền tế bào máu và các tế bào của hệ thần kinh trung ương. IL-6 được sản sinh bởi nhiều loại tế bào khác nhau và hoạt động như một cytokine tiền viêm và kháng viêm. Để có thể biểu hiện hiệu quả trong hệ prokaryote, gen IL-6 của gà đã được tách dòng được tối ưu hóa bằng chương trình Optimum Gene Codon Optimization Analysis và hệ số thích ứng codon đã tăng lên 0,89.
6p
trinhthamhodang
24-10-2019
53
2
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Cytochrome P450 (P450) 4X1 is one of the so-called ‘orphan’ P450s with-out an assigned biological function. Codon-optimized P450 4X1 and a number of N-terminal modified sequences were expressed inEscherichia coli. Native P450 4X1 showed a characteristic P450 spectrum but low expression inE. coliDH5acells (
12p
galaxyss3
07-03-2013
40
2
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