![](images/graphics/blank.gif)
Oxidized protein hydrolase
-
Esterases are often overexpressed in cancer cells and can have chiral specificities different from that of the corresponding normal tissues. For this reason, ester prodrugs could be a promising approach in chemotherapy.
14p
virose2711
25-09-2020
9
1
Download
-
Cancer cell esterases are often overexpressed and can have chiral specificities different from that of the corresponding normal cells and can, therefore, be useful targets for activating chemotherapeutic prodrug esters. Prodrug esters are inactive compounds that can be preferentially activated by esterase enzymes.
13p
vilisa271
22-09-2020
8
1
Download
-
A100-kDa proteinwith endoglucanase activitywas purified from Triton X-100 extract of cells of the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius. The enzyme exhibited activity towards carboxy methyl cel-lulose and oat spelt xylan with pH and temperature optima of 4 and 80C, respectively. Cloning and nucleotide sequence analysis of the corresponding gene (celB) revealed an ORF encoding a preprotein of 959 amino acids which is consistent with an extracellular localization.
10p
tumor12
20-04-2013
32
1
Download
-
The cellobiohydrolase Pc_Cel7D is the major cellulase produced by the white-rot fungusPhanerochaete chrysosporium, constituting 10% of the total secreted protein in liquid culture on cellulose. The enzyme is classified into family 7 of the glycoside hydrolases and, like other family members, catalyses cellulose hydrolysis with net retention of the anomeric carbon configuration.
0p
awards
06-04-2013
28
1
Download
-
The Mycobacterium tuberculosisgenome contains an unusually high number of proteins involved in the metabo-lism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c was overexpressed inE. colias inclusion bodies and refolded. Rv1399c efficiently hydro-lyzes soluble triacylglycerols and vinyl esters.
0p
awards
05-04-2013
46
1
Download
-
Brassica junceachitinase is an endo-acting, pathogenesis-related protein that is classified into glycoside hydrolase family 19, with highest homology (50–60%) in its catalytic domain to class I plant chitinases. Here we report X-ray structures of the chitinase catalytic domain from wild-type (apo,as well as with chloride ions bound) and a Glu234Ala mutant enzyme, solved by molecular replacement and refined at 1.53, 1.8 and 1.7 A˚ resolution, respectively.
9p
galaxyss3
19-03-2013
37
2
Download
-
A cDNA corresponding to theAt3g26690 3 gene, which encodes a Nudix protein (AtNUDT13) with predicted mitochondrial localization, was iso-lated from an Arabidopsis thalianalibrary. The 202 amino acid AtNUDT13 polypeptide was overexpressed inEscherichia coliand purified to homo-geneity.
9p
media19
04-03-2013
19
1
Download
-
Although bacteriophage T5 is known to have lytic proteins for cell wall hydrolysis and phage progeny escape, their activities are still unknown. This is the first report on the cloning, expression and biochemical charac-terization of a bacteriophage T5 lytic hydrolase.
14p
vinaphone15
28-02-2013
36
1
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)