PCR amplified fragments

This study aimed to develop a method using DNA barcode fragments to identify the hybrid Eucalyptus UP99 and UP95. The total genomic DNA was extracted from leaf samples of UP99 and UP95 and was used to amplify the DNA barcodes (matK, rbcL, trnH-psbA, ITS and ITS2) by PCR.

11p vikissinger 21-12-2023 6 1   Download

We present a case of sporotrichosis diagnosed by molecular biology combined with traditional methods. Fungal DNA was extracted from skin tissues of the patient by fungal DNA extraction kit. The PCR amplified fragments were compared on GenBank website, and the pathogenic fungi were identified.

4p viintuit 26-09-2023 1 0   Download

In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands.

10p nguaconbaynhay5 16-05-2020 11 0   Download

Six isolates of chickpea collar rot fungus of Sclerotium rolfsii were collected from different locations of Maharashtra were investigated for genetic diversity under present experiment. We employed five SSR of MB- series to construct a genotype-specific DNA fragment profile of field isolates of this fungus. The PCR amplified product of each primer was resolved on 10 % Polyacrylamide gel electrophoresis. The 5 SSR primers screened produced a total of 60 reproducible and scorable amplicons. The size of amplicons produced ranged from 115bp to 800bp.

8p chauchaungayxua5 05-05-2020 17 2   Download

Present study was conducted with the objectives to identify single nucleotide polymorphism in sperm associated antigen 11 B(SPAG11B) gene and to analyze association between identified polymorphism with conception rate in Murrah bulls in ICAR-National Dairy Research Institute (NDRI) herd, Karnal. A 373 base pair region covering partial intron 2, exon 3 and partial intron3 of bovine SPAG11 gene was amplified using genomic DNA extracted from eighty six Murrah bulls and genotyped using sequencing and polymerase chain reaction- restriction fragment length polymorphisms (PCR-RFLP) methods.

7p cothumenhmong3 22-02-2020 19 0   Download

Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in pJET1.

9p chauchaungayxua3 07-02-2020 15 2   Download

A study was undertaken to isolate, identify and characterize the rice blast pathogen M. grisea from different rice growing regions. In the present study, ten isolates of M. grisea were categorized into different groups based on colony colour and texture. PCR was performed to identify the M. grisea isolates using the universal primers of 18S (ITS 1) and 28S (ITS 4). The PCR reaction allowed amplifying the fungal ITS fragments of 550 bp. All the isolates had the expected specific size of 550 bp which depicts molecular based confirmation of M. grisea.

9p chauchaungayxua3 07-02-2020 12 1   Download

PCR was employed to establish association of begomovirus through amplification of geminivirus specific PCR product. In order to determine the complete nucleotide sequence of DNA-A component of HgYMV, several universal primers/ abutting primers and specific primers available in the literature were tried to amplify full length DNA-A of 2.7 kb. The amplification of full length of DNA-A component of HgYMV was achieved with all the primers such as AC-abut and AV-abut, HgYMVAF and HgYMVAR and HYMVA1500F and HYMV-A1500R.

21p nguaconbaynhay3 07-02-2020 13 1   Download

The present study was carried out for investigation of polymorphism of FecB and POU1F1 gene in Assam Hill goat. Blood samples pertaining to 80 randomly selected Assam Hill goats having kidding history of single as well as multiple births maintained at three field units viz., Batabari, Nahira and Tetelia under “AICRP on Goat Improvement” were utilized. DNA was extracted using modified phenol chloroform extraction procedure. The quantity and quality of extracted DNA were assessed using spectrophotometry and agarose gel electrophoresis.

9p cothumenhmong1 11-12-2019 6 0   Download

The fragmentation of DNA in historical specimens is very common, so obtaining sequences that allow molecular identification and the study of diversity is quite challenging. In this study, we used preserved and fresh specimens of the fruit fly genus Anastrepha, a genus of economic impact of fruit crops of the Neotropic.

12p trinhthamhodang1 14-11-2019 18 0   Download

Cassava (Manihot esculenta Crantz) is one of the most important crops in Vietnam for providing food, starch sources, and raw materials for the production of bio-ethanol and other purposes. Cassava bacterial blight (CBB) disease, caused by Xanthonomas axonopodis pv. manihotis (Xam), is one of the most important factors affecting cassava production in Vietnam. A rapid and sensitive molecular tool is required to support the traditional method, primarily based on biochemical reactions, that was considered less sensitive and time-consuming.

6p caygaocaolon1 13-11-2019 25 1   Download

Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps.

9p caplock2711 19-02-2019 27 0   Download

To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis.

8p caplock2711 19-02-2019 31 0   Download

Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR).

5p aquaman27 15-01-2019 16 1   Download

The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes however amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b).

12p vimb123 11-01-2019 15 1   Download

Nghiên cứu được tiến hành với mục tiêu nhằm xây dựng các phương pháp thuận tiện và có độ lặp lại cao để xác định các loài sâm thuốc chi Panax và nguồn gốc của chúng. Và nghiên cứu áp dụng phương pháp giải trình tự, PCR-Restriction Fragment Length Polymorphic (PCR-RFLP) trên vùng ITS và 18S, và Random Amplified Microsatellite (RAMS).

7p hanh_tv11 15-01-2019 65 2   Download

In this study, we designed two PCR primers to amplify the region containing rs6548238. The AvaI restriction enzyme was selected to detect the SNP polymorphism. Sequencing the resulting bands validated the RFLP method. In conclusion, RFLP method is simple, sensitive and cost effective for genotyping of the TMEM18 rs6548238 polymorphism; thus, this method is most convenient for SNP analysis in large-size samples from Vietnamese population.

7p jangni2 19-04-2018 26 0   Download

PCR-RFLP analysis of beta-lactoglobulin gene locus was carried out on 110 DNA samples of Murrah buffaloes in the present study. A 262 bp fragment enclosing from exon IV to intron IV in b-lg gene was amplified with specific primers. All the 110 DNA samples resulted in 262 bp product on amplification. The PCR products were subjected for digestion with Pst1,EcoRI, HindIII and Hae III enzyme. PCR products were not digested by PstI, EcoRI and HindIII. PCR products when digested with HaeIII enzyme resulted in monomorphic banding pattern in all the samples.

4p tieutuvotam 18-02-2013 64 5   Download

This method is real-time PCR, which can be a qualitative or quantitative assay since amplification and detection of amplified products occur simultaneously. Then, typing is performed. Typing is primarily used for epidemiologic investigations, for studies on pathogenesis such as multiple serotype infections, for unusual or especially severe infections, or for treatment approaches such as high titer γ-globulin. The nucleotide sequences from these fragments were determined by a DNA auto sequencer with fluorescent dideoxy chain terminators.

321p ti_du_hoang 27-08-2012 69 5   Download

More discrete sequence alterations rely heavily on the use of the PCR, which allows rapid gene amplification and analysis. Moreover, PCR makes it possible to perform genetic testing and mutational analysis with small amounts of DNA extracted from leukocytes or even from single cells, buccal cells, or hair roots. Screening for point mutations can be performed by numerous methods (Table 62-9); most are based on the recognition of mismatches between nucleic acid duplexes, electrophoretic separation of single- or double-stranded DNA, or sequencing of DNA fragments amplified by PCR.

10p konheokonmummim 03-12-2010 64 2   Download