PCR array
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Part 2 book "PCR Technology - Current innovations" includes content: Assay design for Real-Time qPCR, primer design for large scale multiplex PCR and arrayed primer extension, hypothesis driven approaches to multivariate analysis of qPCR data, Experiment Design, data management, and univariate statistical analysis of gene expression data obtained by real time quantitative PCR,.... and other contents.
261p oursky06 17-10-2023 3 1 Download
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Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.
116p oursky05 20-09-2023 3 2 Download
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A higher number of interferon-related genes were found to be downregulated in the cells infected with DkPen compared to WSN. On the other hand, significant differences in the expression profiles of interferon response genes were observed in the cells expressing viral PA protein. In particular, avian influenza PA protein was found to cause more aggressive changes on the transcript levels. Human and avian influenza A viruses cause a substantial change in interferon response gene expression in HEK293 cells.
16p thiencuuchu 27-11-2021 9 1 Download
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The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gainof-function screens are less common, although some reports have utilized arrayed viral expression libraries or the CRISPR activation system.
12p visilicon2711 20-08-2021 13 1 Download
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Several techniques have been tailored to the quantification of microRNA expression, including hybridization arrays, quantitative PCR (qPCR), and high-throughput sequencing. Each of these has certain strengths and limitations depending both on the technology itself and the algorithm used to convert raw data into expression estimates.
13p vioklahoma2711 19-11-2020 8 1 Download
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Survivin, a member of the family of inhibitor of apoptosis proteins, functions as a key regulator of mitosis and programmed cell death. YM155, a novel molecular targeted agent, suppresses survivin, which is overexpressed in many tumor types. The aim of this study was to determine the antitumor activity of YM155 in SK-NEP-1 cells.
13p vijisoo2711 30-09-2020 7 1 Download
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Especially in human tumor cells, the osteopontin (OPN) primary transcript is subject to alternative splicing, generating three isoforms termed OPNa, OPNb and OPNc. We previously demonstrated that the OPNc splice variant activates several aspects of the progression of ovarian and prostate cancers.
11p virose2711 22-09-2020 14 2 Download
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High temperature stress is one of the most detrimental abiotic stresses which adversely affect productivity of maize (Zea mays L.) in tropics and subtropics. Plants respond to high temperature stress by regulating expression of an array of genes, heat shock proteins (HSPs) being one of them. Owing to highly differential expression of HSPs in various crop species under high temperature stress, these could be considered as key stress responsive genes.
12p nguathienthan2 26-12-2019 14 0 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR
10p panasonic06 25-12-2011 59 2 Download