PCR as a rapid diagnostic tool

Dermatophytes are a group of closely related keratinophilic fungi that can invade keratinized humans and animals tissues such as skin, hair and nails causing dermatophytosis. They are an important cause of superficial fungal infection. Conventional methods like potassium hydroxide (KOH) direct microscopy and fungal culture lacks the ability to make an early and specific diagnosis, as it takes about four weeks for the culture to grow on Sabourauds dextrose agar.

5p kethamoi4 18-04-2020 16 0   Download

Diagnosis and management of patients with tuberculous pleuritis needs a rapid and sensitive diagnostic tool. Our aim was to diagnose cases of tuberculous pleuritis from patients employing ZN smear, culture (LJ & MGIT) and to compare with PCR. Pleural fluid collected from 36 patients with clinical and/or radiological suspicion of TB pleuritis was included in this study. Pleural fluid collected from nine non-tuberculous pleuritis cases was included as negative controls.

5p caygaocaolon4 04-04-2020 25 0   Download

Avian infectious bronchitis (IB) is one of the major economically important, worldwide distributed and highly contagious and acute upper‐ respiratory tract disease of poultry caused by avian infectious bronchitis virus (IBV) belonging to family Coronaviridae and affects both galliform and nongalliform birds. IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems.

15p caygaocaolon3 27-02-2020 28 0   Download

Corynebacterium pseudotuberculosis, a Gram positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic debilitating disease of sheep, goats and other warm blooded animals. Definite diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, the present study was undertaken with the aim of evaluating a SYBR Green real time PCR assay targeting ABC transporter gene for the detection of C.

6p cothumenhmong1 08-12-2019 22 0   Download