Pcr in diagnosis

The study "Identification of porcine circovirus type 2 (PCV2), type 3 (PCV3) and porcine parvovirus (PPV) in swine by multiplex PCR test" aimed to simultaneously detect three important viruses reported to be involved in the reproductive problems of sows. A multiplex PCR (mPCR) test was developed to provide rapid diagnosis of porcine circovirus type 2 and 3 (PCV2, PCV3) and to illustrate parvovirus (PPV) prevalence in sow herds.

7p tieuvulinhhoa 22-09-2022 3 1   Download

A rapid and reliable multiplex RT-PCR assay for differential diagnosis of NA-PRRSV, EU-PRRSV and HP-PRRSV strains in the study "Multiplex RT-PCR assay to differentiate genotypes of porcine reproductive and respiratory syndrome virus in swine" is quite useful for routine diagnosis of PRRS in swine farms.

6p tieuvulinhhoa 22-09-2022 3 1   Download

The aim of our study is to test procalcitonin (PCT) as surrogate marker of identification of Candida spp. by blood culture (BC) and real-time-polymerase chain reaction (PCR), whether alone or in association with bacteria, in septic patients. Methods: We performed a single-centre retrospective study. We reviewed the clinical charts of patients with a diagnosis of severe sepsis or septic shock treated at our general intensive care unit from March 2009 to March 2013.

9p viisaacnewton 26-04-2022 4 1   Download

Parasites of the family Spirorchiidae cause disease and mortality in marine and freshwater turtles; two species, Hapalotrema mistroides and Neospirorchis sp., are reported in the resident population of loggerhead turtles of the Mediterranean Sea, with the first being the most widespread.

5p vigalileogalilei 28-02-2022 10 1   Download

Sequencing errors are key confounding factors for detecting low-frequency genetic variants that are important for cancer molecular diagnosis, treatment, and surveillance using deep next-generation sequencing (NGS). However, there is a lack of comprehensive understanding of errors introduced at various steps of a conventional NGS workflow, such as sample handling, library preparation, PCR enrichment, and sequencing. In this study, we use current NGS technology to systematically investigate these questions.

15p vigalileogalilei 27-02-2022 9 2   Download

Campylobacter fetus subsp. venerealis (Cfv) is the pathogen responsible for Bovine Genital Campylobacteriosis (BGC), a venereal disease of cattle associated with impaired reproductive performance. Although several PCR assays were developed to identify this pathogen, most of them are still poorly evaluated in clinical samples.

9p vigandhi 23-02-2022 4 1   Download

Dermatophytosis in calves is a major public and veterinary health concern worldwide because of its zoonotic potential and associated economic losses in cattle farms. However, this condition has lacked adequate attention; thus, to develop effective control measures, we determined ringworm prevalence, risk factors, and the direct-sample nested PCR diagnostic indices compared with the conventional methods of dermatophytes identification.

15p vigandhi 23-02-2022 9 1   Download

Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. A real-time fluorescence PCR assay has been developed for the detection of PCV3, with high sensitivity and specificity, exhibiting good performance in detecting clinical samples.

5p vigandhi 23-02-2022 7 1   Download

The detection of epidermal growth factor receptor (EGFR) mutations in plasma cell-free DNA (cfDNA) is an auxiliary tool for the molecular diagnosis of non-small cell lung cancer (NSCLC), especially when an adequate tumor tissue specimen cannot be obtained.

7p lazzaro 30-12-2021 5 0   Download

In this study, formalin-fixed, paraffin-embedded (FFPE) tissue samples were collected from 30 lung cancer patients and 30 patients suffering from non-cancerous pulmonary diseases. The methylation level of SHOX2 was evaluated in two CpG-riched regions of the promoter by using qMSP.

6p meyerowitz 25-12-2021 4 0   Download

In oncology, liquid biopsy is of major relevance from theranostic point of view. The searching for mutations in circulating tumor DNA (ctDNA) in case of colorectal cancers (CRCs) allows the optimization of patient care. In this context, independent of mutation status biomarkers are required for its detection to confirm the presence of ctDNA in liquid biopsies.

12p vimahuateng 26-11-2021 11 0   Download

The clinical signs observed in the ducks characterized by coryza, conjunctivitis, respiratory rales and sneezing. The post mortem lesions in lungs were suggestive of chronic respiratory disease (CRD). On culturing the clinical samples, no bacteria could be isolated. Lung tissue was processed and subjected to polymerase chain reaction (PCR) targeting Mycoplasma gallisepticum species. Samples revealed amplicon size of 183 bp.

5p chauchaungayxua11 23-03-2021 6 0   Download

An experiment was designed to detect garlic bulb associated viruses through RTPCR and to evaluate the response of genotypes against viruses under field condition. The present study was conducted at Bihar Agricultural University, Sabour, Bhagalpur, Bihar during 2019-20 to study the incidence of bulbassociated Potyviruses in garlic through molecular diagnosis. In order to confirm the presence of virus in stored garlic, RT-PCR based assay was used. The suspected leaves of genotypes were tested for the virus.

8p chauchaungayxua11 23-03-2021 15 1   Download

It is very rare for clear cell sarcomas (CCS) to arise in the bone. During diagnosis, it is important to distinguish primary CCS of bone from bone metastasis of melanoma because this difference fundamentally changes the therapeutic options. Recently, characteristic fusion genes of CCS have been detected using reverse transcription polymerase chain reaction (RT-PCR) or direct sequencing which allowed to distinguish CCS from melanoma.

9p vivermont2711 28-01-2021 4 0   Download

The polymerase chain reaction (PCR) for Actinobacillus pleuropneumonia (App), Haemophilus parasuis (Hps), Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bb) were performed in pure colonies isolated from 114 lung specimens with lesions collected from the Vissan slaughterhouse in Ho Chi Minh City from July 2018 to May 2019

6p larachdumlanat129 19-01-2021 9 1   Download

A 2 year old male Jamunapari goat weighing 30 kilograms was presented with complaints of chronic diarrhea, progressive weight loss and poor response to therapy. Faecal sample was collected aseptically and subjected for the presence of Mycobacterium avium subspecies paratuberculosis (MAP) by Ziehl-Neelsen staining and polymerase chain reaction (PCR).

4p gaocaolon9 22-12-2020 6 1   Download

In this study, 100 specimens from tuberculosis confirmed patients and suspected patients who were treated in Hai Duong Tuberculosis and Lung Disease Hospital to perform 3 simultaneous M. tuberculosis detection methods as multiplex PCR assay, direct sputum smear (AFB) and bacterial culture (BK).

7p tamynhan9 02-12-2020 11 1   Download

The present study was conducted in Veterinary Clinical Complex, College of Veterinary Science, Tirupati over a period of nine months to diagnose the Babesia infection. 235 dogs suspected for babesiosis were collected and subjected for diagnosis using conventional and molecular techniques.

6p trinhthamhodang1216 19-11-2020 7 1   Download

Rabies is a highly fatal viral infection of central nervous system affecting all warm blooded animals including humans. The virus belongs to the Rhabdoviridae family, genus Lyssa virus. Several techniques have been standardized internationally for the diagnosis of rabies. This study was designed to assess the efficacy of Seller's staining test (SST) and Direct Fluorescent Antibody Test (dFAT) for diagnosis of rabies antigen, Mouse Innoculation Test (MIT) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) were used as the Gold Standards.

7p trinhthamhodang1215 23-09-2020 5 0   Download

Infectious Bursal Disease (IBD) is an economically important and contagious viral disease of poultry. An attempt was made for development of a synthetic peptide based diagnostic reagent. Cell culture adapted viral antigen was prepared by successful passage on CEF and then ultracentrifugation to get partially purified virus. Confirmation of the virus in was done by RT- PCR. The antigen was then used for the production of hyper immune sera in rabbits. Sera samples were also collected from known IBD vaccinated and non-vaccinated birds.

10p trinhthamhodang1215 23-09-2020 7 0   Download