Pcr in diagnosis

Typhoid fever remain a leading cause of morbidity, mortality and economic loss regardless of age or gender in developing countries. A sensitive and specific test is urgently needed for the accuracy of the diagnosis. The clinical pictures is commonly misleading with other ferial diseases and diagnosis depends mainly on the laboratory. By using molecular method especially nested PCR it provides the highest sensitivity and specificity.

9p nguaconbaynhay3 07-02-2020 11 0   Download

Acinetobacter, has been identified as an important pathogen in nosocomial outbreaks with high levels of emerging drug resistance. So the present study was conducted in Acinetobacter spp to find out the utility of the multiplex PCR assay, which may be used as a useful technique in the early detection & prevention of NDM-1 gene harbouring in clinical isolates taken from the patients coming to the tertiary care hospital.

5p angicungduoc5 12-06-2020 5 0   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Real-time reverse-transcription PCR in the diagnosis of influenza A (H1N1)v in intensive care unit adult patients...

2p coxanh_6 29-10-2011 39 3   Download

The aim of the present study is to identify the presence of Epestein Barr virus (EBV), Human Herpes virus 6 (HHV6)and Human Herpes virus 7 (HHV7) by molecular methods in newly diagnosed ALL in children and to correlate their presence with clinical and immunophenotypes of ALL. The present study included 60 children at the time of diagnosis of ALL before start of the therapy and 60 healthy cross match age and sex children as a control group. The study of EBV, HHC6 and HHV7 was carried out by real time polymerase chain reaction (PCR).

12p kequaidan2 11-12-2019 6 0   Download

Despite Fowl typhoid (FT) eradication in commercial poultry of some parts of the world, outbreak threats are not eliminated in poultry of developing countries. In the current study, we reported molecular identification of Salmonella gallinarum in Indian isolates from backyard poultry by a multiplex PCR assay. Boiled culture lysates of Salmonella Gallinarum isolates (n=2) from an outbreak showed positive amplifications of glgC and speC genes in duplex PCR with 174 bp and 252 bp products, respectively.

5p chauchaungayxua3 07-02-2020 7 0   Download

Diagnosis of disease is the backbone of control and treatment in veterinary field. In addition to the antemortem and post mortem methods, currently several laboratory-based tools and technique are also being used for early diagnosis. Since few decades, polymerase chain reaction (PCR) has emerged as the most preferred molecular diagnostic technique for disease diagnosis due to its high specificity.

8p caygaocaolon2 14-03-2020 6 0   Download

Leptospirosis is one of the most widespread worldwide zoonotic occupational disease which is widely prevalent in various states of India, especially in Tamil Nadu. The mortality in severe disease ranges from 5-40%. Early diagnosis and treatment with appropriate antibiotics contributes to a reduction in the mortality rate. Hence this study was done with the objective of evaluating the usefulness of PCR in early diagnosis of human leptospirosis.

5p caygaocaolon4 01-04-2020 11 0   Download

Persistent and chronic diarrhea is difficult to treat, and infection is still the main cause. In this study, we investigate the application value of xTAG gastrointestinal pathogen panel (xTAG GPP) multiplex PCR in the early diagnosis of persistent and chronic diarrhea in children and to understand the epidemiology of intestinal diarrhea pathogens.

7p vimanama2711 28-07-2020 3 0   Download

Rabies is a highly fatal viral infection of central nervous system affecting all warm blooded animals including humans. The virus belongs to the Rhabdoviridae family, genus Lyssa virus. Several techniques have been standardized internationally for the diagnosis of rabies. This study was designed to assess the efficacy of Seller's staining test (SST) and Direct Fluorescent Antibody Test (dFAT) for diagnosis of rabies antigen, Mouse Innoculation Test (MIT) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) were used as the Gold Standards.

7p trinhthamhodang1215 23-09-2020 4 0   Download

important regulators of biological processes in animals and plants. MiRNAs regulate gene expression at the posttranscriptional level by binding to mRNAs and either inhibit translation or modify the stability of the mRNA. Due to the important biological role of miRNAs it is of great interest to study their expression level in the cells. Furthermore, miRNAs have been associated with cancer and other diseases [1] and miRNA expression can help in the diagnosis and prognostic of human disease [2,3].

11p zingzing09 24-04-2013 43 3   Download

Nucleic acid amplification tests (NAATs) have offered hope for rapid diagnosis of tuberculosis (TB). However, their efficiency with smear-negative samples has not been widely studied in low income settings. Here, we evaluated in-house PCR assay for diagnosis of smear-negative TB using Lowenstein-Jensen (LJ) culture as the baseline test. Two hundred and five pulmonary TB (PTB) suspects with smear-negative sputum samples, admitted on a short stay emergency ward at Mulago Hospital in Kampala, Uganda, were enrolled.

8p taisaocothedung 12-01-2013 38 1   Download

Meningitis due to Neisseria meningitidis and Haemophilus influenzae is a common infectious disease among infants and children that can be fatal in the absence of rapid and proper diagnosis and treatment. Therefore, a rapid and sensitive method for diagnosis is a high priority.

5p vivant2711 01-02-2019 12 1   Download

The extraction of genomic DNA from microbial cells plays a significant role in PCR-based applications such as molecular diagnosis, microbial taxonomy, screening of genetically engineered microorganisms, and other such PCRbased applications. Currently, many methods for extraction of genomic DNA from microorganisms have been developed. However, these methods either require hazardous chemicals or consist of time-consuming steps for effective execution.

9p caygaocaolon1 13-11-2019 9 0   Download

Sickle cell anemia is a serious genetic health problem dominated in Tharu community of western Nepal. Molecular methods like PCR and RFLP are the best method to identify Sickle c ell anemia trait. Molecular analysis needed many steps and expensive chemicals and Kits. The aim of this research was to develop a simple and cheapest method to process from whole blood sample for the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents and Kits.

8p nguathienthan1 27-11-2019 20 0   Download

Brucellosis still remains an infectious, highly contagious and re-emerging endemic zoonosis especially in the Mediterranean and Middle-East regions of the world involving many countries including India where it constitutes occupational hazard (Shakerian et al., 2013 and Khamesipour et al., 2014). It also poses a serious threat to livestock economy by causing abortion, loss of offspring, infertility and reduction in milk yield.

6p chauchaungayxua1 04-12-2019 7 0   Download

Corynebacterium pseudotuberculosis, a Gram positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic debilitating disease of sheep, goats and other warm blooded animals. Definite diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, the present study was undertaken with the aim of evaluating a SYBR Green real time PCR assay targeting ABC transporter gene for the detection of C.

6p cothumenhmong1 08-12-2019 10 0   Download

Papaya ring spot virus (PRSV) is the most destructive disease of Papaya, responsible for yield reduction of Papaya all over the world. PRSV exhibited different types of symptoms on Papaya plant in the field like ringspot , vein clearing, vein banding, chlorosis of younger leaves, filiformy, shoestring, mosaic, mottling, stunted growth etc. Molecular diagnosis of PRSV was done with the help of gene specific primers through PCR. Gel electrophoresis results show a 300bp clear band which confirms the presence of PRSV in the plants. Sequencing was done and phylogenetic tree was made.

8p cothumenhmong1 08-12-2019 6 0   Download

A total of 52 suspected dogs with symptoms of harsh, chronic non-productive coughing, fever and anorexia were examined for tuberculosis in the Teaching Veterinary Clinical Complex, Guwahati, Assam. SIT carried out by injecting 0.1ml of tuberculin (2TU, 5TU and 10TU) at either the skin of the thorax at the level of the costochrondral junction or the skin on the medial aspect of the hind limb in the thigh region and blood was collected aseptically for IFN-γ assay. Out of which the 2 dogs were found positive for TST and 3 dogs were positive for IFN-gamma assay.

6p nguathienthan2 19-12-2019 3 0   Download

Avian infectious bronchitis (IB) is one of the major economically important, worldwide distributed and highly contagious and acute upper‐ respiratory tract disease of poultry caused by avian infectious bronchitis virus (IBV) belonging to family Coronaviridae and affects both galliform and nongalliform birds. IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems.

15p caygaocaolon3 27-02-2020 15 0   Download

Bacterial meningitis is more common in the neonatal period than any other time in life; however, it is still a challenge for the evidence based diagnosis. Strategy for identification of neonatal bacterial meningitis pathogens is presented by evaluating three different available methods to establish evidence-based diagnosis for neonatal bacterial meningitis.

8p vichengshin2711 29-02-2020 17 0   Download