Pcr in diagnosis

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  • Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Real-time reverse-transcription PCR in the diagnosis of influenza A (H1N1)v in intensive care unit adult patients...

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  • important regulators of biological processes in animals and plants. MiRNAs regulate gene expression at the posttranscriptional level by binding to mRNAs and either inhibit translation or modify the stability of the mRNA. Due to the important biological role of miRNAs it is of great interest to study their expression level in the cells. Furthermore, miRNAs have been associated with cancer and other diseases [1] and miRNA expression can help in the diagnosis and prognostic of human disease [2,3].

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  • Nucleic acid amplification tests (NAATs) have offered hope for rapid diagnosis of tuberculosis (TB). However, their efficiency with smear-negative samples has not been widely studied in low income settings. Here, we evaluated in-house PCR assay for diagnosis of smear-negative TB using Lowenstein-Jensen (LJ) culture as the baseline test. Two hundred and five pulmonary TB (PTB) suspects with smear-negative sputum samples, admitted on a short stay emergency ward at Mulago Hospital in Kampala, Uganda, were enrolled.

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  • The sensitivity of Polymerase Chain Reaction (PCR) makes it a potential diagnostic test for detection of M. tuberculosis in samples with low bacillary load. Aim: To assess the efficiency of PCR as compared to routine diagnostics in detection of M. tuberculosis from sputum samples of suspects referred to a tuberculosis clinic and those identified during a morbidity survey. Methods: Respiratory samples (sputum with or without saliva) from 144 individuals were examined by PCR, using MPB64 primers, culture and microscopy.

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  • In this study, we set up and implement a diagnosis procedure to detect and preliminarily characterized FLT3-TKD mutations in Vietnamese AML patients by digesting FLT3 gene PCR products with EcoRV follwed by polyacrylamide gel electrophoresis.

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  • Chapter 124. Sexually Transmitted Infections: Overview and Clinical Approach (Part 17) Diagnosis Although most genital ulcerations cannot be diagnosed confidently on clinical grounds alone, clinical findings plus epidemiologic considerations (Table 124-7) can usually guide initial management (Table 124-8) pending results of further tests. Clinicians should order a rapid serologic test for syphilis in all cases of genital ulcer and a dark-field or direct immunofluorescence test (or PCR test, where available) for T.

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  • Diagnosis in most cases can be made from clinical presentation with certainty; if the clinician has a strong suspicion. Differentials include papular urticaria, chickenpox, mosquito bite etc. Rarity of cases and lack of suspicion as well as uneventful recovery are the most important causes of missing a case clinically. Though laboratory confirmation depends on direct isolation of virus in cell cultures, Indirect fluorescent assays (IFA), RT-PCR or serum neutralization techniques are also useful.

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  • In Lyme Borreliosis, western blot is the preferred serologic test. Antigen detection tests (antigen capture and PCR), although insensitive, are very specific and are especially helpful in evaluating the seronegative patient and those still ill or relapsing after therapy. Often, these antigen detection tests are the only positive markers of Bb infection, as seronegativity has been reported to occur in as many as 30% to 50% of cases. Nevertheless, active LB can be present even if all of these tests are non-reactive! Clinical diagnosis is therefore required.

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