Phylogenetics

Objectives: This study aims to evaluate the species diversity, distribution pattern and phylogenetic relationships of the genus Megophrys in Vietnam.

28p thebadguys 08-06-2021 15 3   Download

The current investigation was performed with two major objectives: Assessment of BPH resistance of the batch of converged rice cultivars and local rice varieties; Phylogenetic analysis and using marker molecular-based breeding methods to develop the excellent 2-3 cultivars with sustained resistance to BPH for providing in the Mekong River Delta.

27p trinhthamhodang7 31-08-2020 9 2   Download

Structures of cell wall anionic polymers of the strainStrep-tomycessp. VKMAc-2124, a causative agent of potato scab, which is phylogenetically the closest to plant pathogenic speciesS. setoniiandS. caviscabies, were studied. The strain contains threeanionicglycopolymers, viz., a teichuronicacid with a disaccharide repeatingunitfi6)-a-D-Glcp-(1fi4)-b-D-ManpNAc3NAcA-(1fi,ab-glucosylated polymer of 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid (Kdn), and ab-glucosylated 1,5-poly(ribitol phosphate). ...

6p tumor12 22-04-2013 36 4   Download

The phylogenetically conserved eukaryotic translation initi-ation factor 5A(eIF5A) is the onlyknown cellular protein to contain thepost-translationallyderivedaminoacidhypusine [N e -(4-amino-2-hydroxybutyl)lysine]. Both eIF5A and its hypusine modification are essential for sustained cell proli-feration. Normally only one eIF5A protein is expressed in human cells. Recently, we identified a second humanEIF5A gene that would encode an isoform (eIF5A-2) of 84% sequence identity.

10p tumor12 20-04-2013 42 3   Download

Acomprehensive, structural and functional, insilicoanalysis of the medium-chain dehydrogenase/reductase (MDR) superfamily, including 583 proteins, was carried out by use of extensive database mining and theBLASTPprogram in an iterative manner to identify all known members of the superfamily. Based on phylogenetic, sequence, and func-tional similarities, the protein members of the MDR super-family were classified into three different taxonomic categories: (a) subfamilies, consisting of a closed group containing a set of ideally orthologous proteins that perform the same function;...

26p tumor12 20-04-2013 35 5   Download

We report the cloning, expression, pharmacological char-acterization and tissue distribution of a melanocortin (MC) receptor gene in a shark, the spiny dogfish (Squalus acanth-ias) (Sac). Phylogenetic analysis showed that this receptor is anorthologof theMC4 subtype, sharing71%overall amino acid identity with the human (Hsa) MC4 receptor. When expressedandcharacterizedbyradioligandbindingassay for the natural MSH (melanocyte-stimulating hormone) pep-tides a-, b-, and c-MSH, the SacMC4 receptor showed pharmacological properties very similar to the HsaMC4 receptor. ...

9p tumor12 20-04-2013 49 3   Download

Inorganic pyrophosphatase (PPase) is an important enzyme that catalyzes the hydrolysis of inorganic pyro-phosphate (PPi)intoortho-phosphate (P i ). We report here the molecular cloning and characterization of a gene encoding the soluble PPase of the roundworm Ascaris suum. The predicted A. suumPPase consists of 360 amino acids with a molecular mass of 40.6 kDa and a pI of 7.1.

13p fptmusic 16-04-2013 35 3   Download

Phylogenetic evidence is presented that primitively amito-chondriate eukaryotes containing the nucleus, cytoskele-ton, and endomembrane system may have never existed. Instead, the primary host for the mitochondrial progeni-tor may have been a chimeric prokaryote, created by fusion between an archaebacterium and a eubacterium, in which eubacterial energy metabolism (glycolysis and fermentation) was retained.

20p fptmusic 16-04-2013 32 4   Download

Coenzyme F420 is a 5-deazaflavin. Upon reduction, 1,5 dihydro-coenzyme F420 is formed with a prochiral centre at C5. All the coenzyme F420-dependent enzymes investigated to date have been shown to be Si-face stereospecific with respect to C5 of the deazaflavin, despite most F420 -dependent enzymes being unrelated phylogenetically. In this study, we report that the recently discovered F420H2 oxidase from methanogenic Archaea is alsoSi-face stereospecific.

6p fptmusic 12-04-2013 31 3   Download

Analysis of a draft nuclear genome sequence of the diatomThalassiosira pseudonana revealed the presence of 11 open reading frames showing significant similarity to functionally characterized fatty acid front-end desaturases. The corresponding genes occupy discrete chromosomal loca-tions as determined by comparison with the recently published genome sequence. Phylogenetic analysis showed that two of the T. pseudonana desaturase (Tpdes) sequences grouped with proteobacterial desaturases that lack a fused cytochrome b5 domain. ...

12p fptmusic 11-04-2013 32 2   Download

Prion diseases are characterized by the accumulation of a pathogenic mis-folded form of a prion protein (PrP) encoded by the Prnpgene in humans. In the present study in zebrafish, two transcripts and the corresponding genes encoding prion proteins, PrP1 and PrP2, related to human PrP have been characterized with a relatively divergent deduced amino acid sequence, but a well preserved overall organization of structural prion pro-tein motifs.

14p awards 05-04-2013 39 2   Download

We report the cloning and characterization of two melano-cortin receptors (MCRs) from the spiny dogfish (Squalus acanthias) (Sac). Phylogenetic analysis shows that these shark receptors are orthologues of the MC3R and MC5R subtypes, sharing 65% and 70% overall amino acid identity with the human counterparts, respectively. The SacMC3R was expressed and pharmacologically characterized in HEK293cells.

0p awards 05-04-2013 39 3   Download

Molecular phylogeny among catalase–peroxidases, cyto-chromecperoxidases, and ascorbate peroxidases was ana-lysed. Sixty representative sequences covering all known subgroups of class I of the superfamily of bacterial, fungal, and plant heme peroxidases were selected. Each sequence analysed contained the typical peroxidase motifs evolved to bind effectively the prosthetic heme group, enabling per-oxidatic activity.

13p awards 05-04-2013 58 6   Download

An open reading frame (Acc. no. P50740) on theBacillus subtilis chromosome extending from bp 184 997–186 043 with similarity to theidi-2 gene ofStreptomycessp. CL190 specifying type II isopentenyl diphosphate isomerase was expressed in a recombinant Escherichia colistrain. The recombinant protein with a subunit mass of 39 kDa was purified to apparent homogeneity by column chromatog-raphy. The protein was shown to catalyse the conversion of dimethylallyl diphosphate into isopentenyl diphosphate and vice versa at rates of 0.23 and 0.

12p dell39 03-04-2013 44 4   Download

We have cloned and determined the nucleotide sequence of the cDNAcoding for a cellular retinol-bindingprotein type I (CRBPI)from zebrafish. The deduced amino acid sequence of the zebrafish CRBPI showed highest sequence identity (
59%)to the mammalian CRBPIs of the intracellular lipid-binding protein (iLBP)multigene family. Phylogenetic analysis clustered the zebrafish CRBPI to the CRBPI clade. The zebrafish CRBPI gene (rbp1)and CRBPII gene (rbp2) both consist of four exons separated by three introns, iden-tical to all otheriLBP genes in vertebrates....

10p dell39 03-04-2013 46 4   Download

A phylogenetic analysis of more than 350 multicopper oxidases (MCOs) from fungi, insects, plants, and bacteria provided the basis for a refined classification of this enzyme family into laccasessensu stricto (basidiomyc-etous and ascomycetous), insect laccases, fungal pigment MCOs, fungal ferroxidases, ascorbate oxidases, plant laccase-like MCOs, and bilirubin oxidases.

19p inspiron33 26-03-2013 53 4   Download

In insects, the functional molecules responsible for the taste system are still obscure. The gene for a 28.5 kDa protein purified from taste sensilla of the blowflyPhormia reginabelongs to a gene family that includestakeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that the PhormiaTakeout-like protein is most similar to the protein encoded by a member of theDrosophila takeoutgene family, CG14661, whose expression and function have not been identified yet.

11p inspiron33 25-03-2013 42 5   Download

Many branchiopod crustaceans are endowed with extracellular, high-molecular-weight hemoglobins whose exact structural characteristics have remained a matter of conjecture. By using a broad spectrum of techniques, we provide precise and coherent information on the hemoglobin of one of the phylogenetically ‘oldest’ extant branchiopods, the tadpole shrimp Triops cancriformis.

17p inspiron33 25-03-2013 42 4   Download

Rapid evolution driven by positive Darwinian selection appears in toxins of vipers, scorpions, and marine snails. Although the vast phylogenetic distan-ces between these animals suggest that this phenomenon is common, the recent release of the genome ofNematostella vectensis(Starlet anemone) as a collection of contigs portrays another extreme.

7p inspiron33 25-03-2013 42 3   Download

Five healthy adult female first-generation hybrid tree frogs were produced by interspecific breeding of closely related tree frogsLitoria splendidaand L. caeruleain a cage containing large numbers of males and females of both species. Phylogenetic analysis of mitochondrial DNA sequences estab-lished the female parent to be L. splendida.

9p inspiron33 25-03-2013 37 4   Download