Plant micropropagation

Ebook "Disposable bioreactors (Advances in biochemical engineering/biotechnology, Volume 115)" includes eight contributions from renowned experts, is to give an up-to-date overview of the main disposable bioreactors, their working principles, characteristics, known engineering aspects, and potential applications. R. Brecht discusses disposable bioreactor technology development in pharmaceutical glycoprotein manufacturing from the view of the applier (CMO).

228p manmanthanhla0201 26-02-2024 7 1   Download

In the micropropagation of difficult-to-root plants, the low rates of rooting cause high economic losses. The local apple (Malus domestica Borkh.) cultivar #67, which is highly resistant to fire blight disease, has been shown to have low rooting success. Nanoparticles loaded with auxins could increase the rooting success with their physicochemical properties.

12p lyhuyenthu 31-01-2023 5 2   Download

Continued part 1, part 2 of ebook "Plant biology and biotechnology (Volume II: Plant genomics and biotechnology)" provide readers with content about: applications of triploids in agriculture; improving secondary metabolite production in tissue cultures; somaclonal variation in micropropagated plants; in vitro conservation of plant germplasm;... Please refer to the part 2 of ebook for details!

375p langmongnhu 14-12-2022 10 3   Download

Green rose (Rose L.) is a beautiful, precious flower that is loved by many people all over the world. However, the source of seedlings of the ornamental flower variety is very limited. This study was conducted to rapidly propagate green roses by tissue culture.

8p vilamborghini 12-10-2022 9 4   Download

In this study the effectiveness of plant growth regulators on micropropagation, total phenol content and metabolic profiles of Melissa officinalis L., important medicinal herb was assessed. The stem segments derived from one-month old in vitro germinated seedlings were used for initial explants. Of the eight different nutrient media tested the most favorable for micropropagation were found to be MP2 (MS medium enriched with 1mg/L BAP and 0.1 mg/L IBA) and MP3 (MS supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA), which yielded 3.25- 4.0 shoots per explant within 4 weeks of culture.

5p mudbound 10-12-2021 16 1   Download

Using chemicals known as inhibitors of chromatin remodeling enzymes is a useful approach in understanding the regulation machinery that contributes to the maintenance of or conversion to a pluri- and totipotent states of the cells in multicellular organisms. They can serve as tools to reveal the molecular and cellular mechanisms required for reprogramming. A deeper understanding of reprogramming pathways that drive the cell into a dedifferentiated state might help us enhance plant micropropagation and green biotechnology applications.

9p tudichquannguyet 29-11-2021 10 1   Download

Tieu Hong banana variety (Musa acuminata) is one of the most popular bananas in Vietnam because they have not only beautiful colour, delicious taste but also high nutrition, and significantly economical value. Nowadays, this banana variety is primarily propagated in wide-scale by micropropagation which is effective with a high propagation coefficient, thus, that can meet the increasing demand of people to enjoy it.

6p retaliation 18-08-2021 8 2   Download

Roses are one of the most popular fl owers in the world because of their beauty, color, aroma as well as their various uses and Red Eden rose is one of the most popular hybrid roses. Results of the study showed the most optimal medium for shoot multiplication, which was MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 0.5 mg/l of BA. The optimal medium for growth and fl owering was ½MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 30 μM of AgNO3 . However, NAA at all tested concentrations did not show any eff ect on in vitro plant growth.

7p nguaconbaynhay12 03-06-2021 23 2   Download

Cunninghamia lanceolata (C. lanceolata) is the main fast-growing timber species in southern China. As an alternative to conventional lighting systems, LED has been demonstrated to be an artificial flexible lighting source for commercial micropropagation.

12p vijichea2711 28-05-2021 10 1   Download

Chickpea is best nutritional crop in leguminous species, and biotic and abiotic stress affects the yield loss around 50 to 60 % .For preventing yield loss from this stress, it is necessary to developed high yield, qualitative variety of chickpea. In vitro methods are best technique for regeneration and development of transgenic variety of chickpea. In this investigation research to determine chickpea in vitro micropropagation was studied in genotypes; of chickpea JG62, JG63 JG315, VIJAY and Bhupdachana with explants immature cotyledonary and embryonic axis.

10p cothumenhmong11 11-05-2021 5 1   Download

Production of bamboo plants by using vegetative propagation is not simple to establish a commercial fields however micropropagation techniques has much ability to achieve the bamboo seedling demands. In vitro cultures of Bambusa balcooa were achieved by using nodal segment as an explant. Eight different combinations of plant growth regulators with MS basal medium were used for shoot induction and multiplication.

7p trinhthamhodang11 27-04-2021 15 2   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation.

6p trinhthamhodang1218 26-02-2021 11 1   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 18 4   Download

In addition, the net photosynthetic rate (Pn) of plants under PA condition was statistically higher and increased over the culture period while Pn of plants under PM condition remained constant. This study demonstrated that MS salts with half concentration of NH4NO3 and photoautotrophic culture method were appropriate for in vitro growth of hydrangea.

11p nguaconbaynhay10 20-02-2021 11 2   Download

The present investigation was emphasized on development of an efficient in vitro micropropagation protocol for high frequency mass multiplication of true-to-type plants and to detect the genetic uniformity of micropropagated plants of Stevia using molecular markers.

13p nguathienthan8 20-10-2020 12 2   Download

An experiment was employed to identify the suitable rooting hormone and a potential hardening medium for gerbera micropropagation techniques. IBA (Indole Butyric Acid) and GA3 (Gibbrelic Acid) are taken for the study of rooting and Sand, Cocopeat, Vermicompost and Vermiculite selected alone or in combinations for the study of hardening in gerbera. Different parameters on root induction were taken similarly morphological and biochemical parameters were studies in hardend plants of gerbera.

9p chauchaungayxua8 03-10-2020 7 1   Download

Plant genetic transformation has become an important biotechnological tool for the improvement of many crops. A solid foundation for the fast development and implementation of biotechnology in agriculture has been provided by achievements in plant tissue culture. Plant tissue culture represents the most promising areas of application at present time and giving an out look into the future.

15p kethamoi7 26-08-2020 21 2   Download

Paphiopedilum orchids, the most popular and rare orchid genera, are used as potted flowers and cut flowers. In nature condition, the orchid population is threatened with extinction due to over-exploitation and altered habitat. Reduction in this orchids via large-scale micropropagation is a better option to reduce the wild harvest, meets the commercial needs as well as conserves this threatened orchid. This orchid can be propagated by germinating seeds under in vitro culture condition; however, they are difficult for micropropagation, especially regenerating plantlets from in vitro culture.

9p nguyenxuankha_bevandan 14-08-2020 13 1   Download

Genetic uniformity of in vitro raised plants is a prerequisite for production of quality planting material of banana. Embryogenic cultures were induced from immature male flower buds of two important cultivars of banana, ‘Rasthali’ (Silk-Musa AAB) and ‘Grand Naine’ (Cavendish-Musa AAA). Cell suspensions were established and regenerated plants were evaluated for their genetic stability using 10 inter simple sequence repeat markers.

11p angicungduoc6 20-07-2020 9 1   Download