Prevalence and molecular detection

The incidence and prevalence of nontuberculous mycobacterial disease is increasing due to enhanced clinician awareness and improved detection methods. The species identification using molecular microbiology techniques allows a better understanding of the differences in pathogenicity and treatment response.

2p viintuit 26-09-2023 0 0   Download

Anaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes.

12p vigandhi 23-02-2022 13 1   Download

Microsporidia are common opportunistic parasites in humans and animals, including rabbits. However, only limited epidemiology data concern about the prevalence and molecular characterization of Enterocytozoon bieneusi and Encephalitozoon spp. in rabbits. This study is the first detection and genotyping of Microsporidia in pet rabbits in China.

8p vigandhi 23-02-2022 8 1   Download

This study was undertaken to know prevalence of major Streptococcus spp. as an etiological agents of bovine mastitis with conventional and molecular methods its antimicrobial resistance patterns.

9p chauchaungayxua11 23-03-2021 12 1   Download

CCD is an autosomal dominant disorder caused by haploinsufficiency of Runt related transcription factor (RUNX2) essential for osteoblast cell commitment and chondrocyte maturation. A mutation of the RUNX2 (Core Binding Factor-α1 gene located at chromosome 6p21) is usually caused CCD. In general, the estimated prevalence of CCD is under diagnosed because of the relative lack of medical complications in comparison to other skeletal dysplasias. No available data on the prevalence of CCD or its characterization in Saudi families.

13p angicungduoc6 20-07-2020 10 2   Download

Lower respiratory tract infections are one of the leading causes of morbidity and mortality in children worldwide. Several viruses can cause bronchiolitis. Respiratory syncytial virus (RSV), parainfluenza viruses (PIV), influenza type A, adenovirus and human metapneumovirus (HMPV) are some of the common viruses found in hospital studies. This study aimed to study the molecular diagnosis of HPMV infections by using a realtime, reverse-transcriptase PCR assay and the evaluation of its prevalence among Egyptian infants clinically diagnosed with acute viral bronchiolitis.

8p kethamoi6 29-06-2020 19 2   Download

This study attempted to determine the prevalence of HBV infection and comparative assessment between serological and molecular diagnoses among 75 samples divided into three groups as treated, untreated and control groups from different cities in Wasit Province, Iraq, during the period from March 2016 to May 2016. The samples were analyzed by ELISA and Real Time – PCR techniques for detecting the prevalence of HBV in patients.

7p chauchaungayxua6 26-06-2020 14 1   Download

The present study was conducted to study the prevalence of the food borne zoonotic pathogen of animal origin, L. monocytogenes by isolation and identification, molecular detection and antibiotic sensitivity pattern from different samples of cattle sources in Aizawl district of Mizoram. A total 200 numbers of sample including cattle faeces (50), raw milk (50) and milk products (100) were collected randomly from different unorganized shop and farms.

15p gaocaolon5 14-06-2020 11 2   Download

Molecular detection of Banana Bunchy Top Virus (BBTV) was carried out using nucleic acid based polymerase chain reaction (PCR) method with six different primer pairs for the six components of BBTV (DNA1 to DNA6) genome. Samples were collected from six banana cultivars exhibiting the characteristic symptoms Banana bunchy top disease from eight districts of Assam viz., Jorhat, Golaghat, Sonitpur, Morigaon, Nagaon, Kamrup Metro, Kamrup Rural and Goalpara.

14p gaocaolon5 14-06-2020 9 3   Download

Bovine tropical theileriosis caused by Theileria annulata is an economically important disease of cattle in tropical and subtropical countries. Conventional diagnostic methods are unable to identify the subclinical and carrier status of this disease. Molecular diagnostic methods are more sensitive in detection of subclinical and carrier condition on the disease. In this study 817 blood samples were collected from cattle of seven different agroclimatic zone of Tamil Nadu state.

7p angicungduoc5 12-06-2020 14 0   Download

Equine theileriosis, being an OIE listed disease mainly requires frequent disease screening and monitoring. The aim of the present study was to monitor the prevalence of theileriosis in horses by using both parasitological method and molecular diagnostic technique based on Equine Merozoite Antigen-5 and 6. In the present study, samples from 248 horses were collected randomly.

6p nguathienthan5 04-06-2020 7 0   Download

Cytomegalovirus infections are endemic worldwide. The most frequently used methods for detecting antibodies in developing world are the Enzyme Linked Immunosorbent Assay. The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific deoxyribonucleic acid fragments is induced from very low concentrations of complex substrates allowing the detection of very low amounts of viral particles.

6p nguathienthan5 04-06-2020 8 1   Download

Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. The objectives of the study were to determine the prevalence of multidrug-resistant Enterobacteriaceae species isolated from the urine samples in a tertiary care hospital and to detect their drug resistance patterns and their genetic characterization.

7p kethamoi5 03-06-2020 9 0   Download

Mycoplasma gallisepticum and Mycoplasma synoviae are important poultry pathogens responsible for high economic losses to the poultry industry in term of mortality, poor weight gain and decrease of feed efficiency in broiler chickens and reduction of egg production in layer chickens and turkeys. Serum plate agglutination (SPA), hemagglutination inhibition (HI) and ELISA are the most common serological techniques used for rapid detection of infection and the adoption of control measures of the diseases.

8p angicungduoc4 26-04-2020 25 0   Download

Japanese encephalitis (JE) is a mosquito borne viral infection of brain. It is prevalent worldwide, most prominent in eastern and southern Asia. Domestic pigs and wild birds are reservoirs of the virus; humans get the disease through mosquito bite that carries the virus. Therefore, the present study was undertaken to uncover the prevalence of antibodies to Japanese encephalitis virus and its nucelic acid in naturally infected pigs of Punjab by commercial indirect ELISA kit and Reverse Trancriptase-PCR (RT-PCR).

6p trinhthamhodang1212 06-04-2020 11 1   Download

Huanglongbing (HLB) or Citrus Greening Disease is a widespread and serious threat to the Citrus growing industry worldwide due to its complexity and destructiveness. On an average, the disease can cause 30 to 100 per cent yield loss around the globe depending on its severity. In Assam, an important factor associated with Citrus decline is the wide spread prevalence of Huanglongbing (HLB) in Khasi mandarin (Citrus reticulata). HLB was detected by molecular methods in Khasi mandarin orchards of Assam state in Northeastern India.

5p caygaocaolon4 04-04-2020 12 1   Download

Tuberculosis is a major public health problem and second largest cause of death among infectious diseases. Rapid diagnosis of tuberculosis and detection of drug resistance is essential for effective disease control. Control of TB is hindered by the lack of a simple and effective diagnostic tool that can be utilised in resource limited settings. Molecular techniques using Real time PCR technology have gained momentum for rapid diagnosis and detection of drug resistance.

8p caygaocaolon2 11-03-2020 15 0   Download

Respiratory tract infections are among the most common infections in India. Multiple etiological agents are involved in the causation of these infections. Swine flu H1N1 is one among the influenza viruses which has great potential to cause epidemics as well as pandemics with great mortality and morbidity. All the suspected cases should be identified at the earliest so that interventions can be taken to prevent the spread of infections and thus contain the outbreaks.

6p caygaocaolon3 27-02-2020 10 1   Download

Avian mycoplasmosis is responsible for heavy economic loses to the poultry industry in India. It results from a complex interaction of various factors like viral, bacterial and housing conditions. Mycoplasma gallisepticum (MG) belongs to class Mollicutes is small in size, lacks bacterial cell wall with minimum genetic information. It is the principle pathogenic agent causing Chronic Respiratory disease (CRD) in chickens and infectious sinusitis in Turkeys and has a worldwide distribution.

6p nguaconbaynhay3 07-02-2020 6 0   Download

The present study was carried out to know the status of haemoprotozoan infection of domestic pigeon in Assam by microscopic examination of blood of pigeons for a period of one year which revealed an overall prevalence of 53.39%. Three species viz. Haemoproteus columbae (29.93%), Plasmodium relictum (21.29%) and Leucocytozoon sp. (2.16%) were identified either in single or mixed infection. According to age, highest prevalence was recorded in adult (61.81%) and lowest in squab (36.25%). Comparatively, infection was recorded higher in females (58.22%) than males (48.79%).

11p nguathienthan2 19-12-2019 9 1   Download