Primer extension

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  • Abstract Epidermal growth factor receptor (EGFR) kinase domain mutations hyperactivate the kinase and confer kinase addiction of the non-small-cell lung cancer (NSCLC) tumor cells. Almost all of these mutations are located within exons 18-21. The -216 single nucleotide polymorphism in the promoter region is associated with increased EGFR production. We present a method for detecting these common mutations in 81 cases of NSCLC.

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  • The term DNA sequencing refers to methods for determining the order of the nucleotides bases adenine,guanine,cytosine and thymine in a molecule of DNA. The first DNA sequence were obtained by academic researchers,using laboratories methods based on 2- dimensional chromatography in the early 1970s. By the development of dye based sequencing method with automated analysis,DNA sequencing has become easier and faster.

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  • Automated DNA sequencing is a core research tool used by almost every research biochemistry lab. It is used to determine the sequence of DNA, or the genetic code, that serves as the blueprint of life for every organism on Earth.

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  • A novel method, based upon primer extension, has been developed for measuring the reopening temperature of a single type of DNA hairpin structure. Two DNA oligo-nucleotides have been utilized and designated as primers 1 and2.Primer1,withits5- and3¢-termini fully comple-mentary to the hairpin flanking sequences, was used to evaluate primer extension conditions, and primer 2, with its 3¢-end competing with the DNA hairpin stem, was used to detect the DNA hairpin reopening temperature.

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  • The immune coagulant fgl2/fibroleukin has been previously shown to play a pivotal role in the pathogenesis of murine and human fulminant hepatitis and fetal loss syndrome. Constitutive expression of fgl2 transcripts at low levels are seen in cytotoxic T cells, endothelial, intestinal and tropho-blast cells, while specific factors (such as virus and cytokines) are required to induce high levels of fgl2 expression in other cell types including monocytes/macrophages.

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  • With SQLite, you’ll discover how to develop a database-backed application that remains manageable in size and complexity. This book guides you every step of the way. You’ll get a crash course in data modeling, become familiar with SQLite’s dialect of the SQL database language, and much more.

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  • The purpose of Pollution Probe’s Mercury Primer is to provide an overview of the presence and effects of mercury in the environment and its impacts on human health. The primer iden- tifies where mercury is being used and released, the risks asso- ciated with exposure to mercury, and ways to help prevent mercury pollution. The primer also describes what governments, businesses and individuals are doing to eliminate the use of mercury and prevent its release to the environment. Pollution Probe has done extensive work on mercury in the envi- ronment for nearly a decade.

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  • Maple is a very powerful computer algebra system used by students, educators, mathematicians, statisticians, scientists, and engineers for doing numerical and symbolic computations. Greatly expanded and updated from the author's MAPLE V Primer, The MAPLE Book offers extensive coverage of the latest version of this outstanding software package, MAPLE 7.0

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