Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro
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A novel method, based upon primer extension, has been developed for measuring the reopening temperature of a single type of DNA hairpin structure. Two DNA oligo-nucleotides have been utilized and designated as primers 1 and2.Primer1,withits5- and3¢-termini fully comple-mentary to the hairpin flanking sequences, was used to evaluate primer extension conditions, and primer 2, with its 3¢-end competing with the DNA hairpin stem, was used to detect the DNA hairpin reopening temperature.
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