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rDNA regions
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Environments with temperatures from 50°C to 80°C are rare in nature and are almost exclusively associated with geothermal regions including hot springs, solar-heated soils and volcanic areas. Thermophilic bacteria already exist and prefer in such habitats.
9p
vimulcahy
18-09-2023
6
3
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In order to accurately identify ginseng species (Panax spp.) that grow naturally in some Northern provinces of Vietnam, we collected 30 natural ginseng samples and used nuclear genetic region (ITSrDNA) for current analysis.
9p
vimulcahy
18-09-2023
4
3
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Inocybe viscida sp. nov., a novel species found in a forest with old trees of Pinus brutia and tertiary relict endemic Liquidambar orientalis in coastal area of southwestern Turkey, is described and illustrated. We present this new species based on morphological and molecular data from nuclear rDNA internal transcribed spacer region (ITS1-5.8SITS2 = ITS) and the portions of nuc 28S rRNA (LSU) gene. Comprehensive description, field photographs, line drawings of the microscopic features and comparisons with phenetically similar taxa and phylogenetically related species are discussed.
11p
lyhuyenthu
31-01-2023
9
2
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The whole repeat unit of 5S rDNA and the internal transcribed spacer (ITS) of four European Donax species were analysed. After amplifying, cloning and sequencing several 5S and ITS units, their basic features and their variation were described.
13p
vihagrid
30-01-2023
11
3
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In this study, we have sequences nuclear gene region (ITS-rDNA) from 9 individuals of Tacca chantrieri André belonging to three natural populations (Song Hinh Nature Reserve, Phu Yen Province; Cuc Phuong National Park, Ninh Binh province and Ba Vi National Park, Ha Noi city) to investigate genetic character and their phylogenetic inference in the genus Tacca. DNA was extracted from the tissue of leaves.
8p
vilamborghini
12-10-2022
7
4
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The phylogenetic relationship of 83 taxa of Santalaceae s.l. was reconstructed based on the molecular data sets including the nuclear ribosomal (small and large subunit rDNA) and the chloroplast rbcL, matK, and trnL-F regions. The Maximum likelihood (ML) and Bayesian inference (BI) methods were used to build the phylogenetic trees.
8p
viellenkullman
13-05-2022
6
1
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Exploring the phylogenetic relationships between taxa provides important information for science. The phylogenetic study of Macrosolen was conducted based on molecular data sets of 27 taxa with five DNA regions including chloroplast rbcL, matK, and trnL-F and nuclear ribosomal (small subunit rDNA and large subunit rDNA) regions to reconstruct the phylogenetic relationship of Macrosolen.
7p
viginnirometty
04-05-2022
17
1
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The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius).
8p
vigandhi
23-02-2022
11
1
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The Mediterranean cereal cyst nematode, Heterodera latipons, is an important plant parasitic nematode that negatively impacts cereal production worldwide, particularly in the Mediterranean Basin. Successful control of cereal cyst nematodes requires knowledge of the nematode species/pathotypes and their biology. However, little information is currently available concerning the phenotypic and genotypic variability of H. latipons populations in Turkey. This study analyzed the morphological/morphometric and genetic characteristics of Turkish H.
12p
dolomite36
30-12-2021
10
1
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Previous studies on the distribution of the Ahaetulla snake across Indonesia only focused on morphological characters without any molecular data. This study was aimed at analyzing phylogenetic relationships among the genus Ahaetulla in Indonesia based on partial mitochondrial DNA sequences of 16 specimens collected from the Sundaland and Lesser Sunda regions. The 12S-rDNA gene was PCR-amplified and sequenced to analyze phylogenetic relationships and to estimate the divergence time between the 2 Ahaetulla populations of the Sundaland and Lesser Sunda regions.
11p
dolomite36
30-12-2021
10
0
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Pluteus and Volvopluteus are two closely related genera of Pluteaceae that comprise saprotrophic fungi. Although the genera have been well-studied in Europe and North and South America, the diversity of Pluteus, mainly Pluteus sect. Pluteus, and Volvopluteus in Turkey is poorly known due to insufficient morphological descriptions and a lack of DNA sequence data. In this study, the diversity of Pluteus sect. Pluteus and Volvopluteus in Turkey is evaluated by employing both morphological features and phylogenetic approaches. A general phylogeny of Pluteus sect.
19p
tudichquannguyet
29-11-2021
8
1
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A molecular key based on the sequence of the internal transcribed spacer (ITS) of the ribosomal cistron was used for the identification of common Trichogramma (Hymenoptera: Trichogramma tidae) species found in agricultural field of Alhassa oasis in Saudi Arabia. The aligned sequences were checked using BLAST program at GenBank. The sequences of the three ITS region were the same and shared identities ranged from 98.3 to 99.4% against all Trichogramma cacoeciae sequences at the GenBank.
8p
quenchua6
15-06-2020
13
2
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Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through ITS1-5.8s-ITS 4 primer series.
10p
trinhthamhodang1213
30-05-2020
8
0
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The present investigation aimed to study and compare the efficiency of non-viable fungal isolates to remove divalent lead (Pb(II)) from aqueous streams. The selected fungal isolates showed identity with Aspergillus caespitosus, Aureobasidium sp. RBSS-303 and Aspergillus favus HF5 as confirmed using gene sequencing of ITS regions of the ribosomal DNA (rDNA).
13p
vijiraiya2711
27-05-2020
12
1
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Recently we found new Pleurotus sp. isolate KKM 1 that grew on wood logs. The experiments were conducted to study its morphological and phenotypic characters of mycelium and basidiocarps of Pleurotus isolate KKM 1 compared with other cultivated mushroom varieties such as P. eous var. APK1, and P. djamor var. MDU1 and P. florida. Pleurotus isolate KKM 1 was confirmed as Pleurotus djamor by ITS sequencing of rDNA region. Thus, the new Pleurotus sp. isolate KKM1 was named as P. djamor isolate KKM1.
9p
trinhthamhodang1213
27-05-2020
11
1
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Genetic variability among the 24 isolates of S. rolfsii was studied by using molecular markers like ITS-PCR and RAPD primers. Amplification of ITS region of rDNA with specific ITS1 and ITS4 universal primers produced approximately 650 to 700 bp in all the isolates of the fungus confirmed that all the isolates obtained are Sclerotium rolfsii and were sequenced. Identity of the isolates was confirmed with sequences of NCBI data base of S. rolfsii.
10p
kequaidan4
05-05-2020
10
1
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Eight native strains of Pseudomonas spp. were isolated from rhizosphere of various sugarcane clones on Pseudomonas agar using standard protocols. These strains were characterized for antagonistic traits on red rot pathogen in vitro and Plant growthpromoting (PGP) activities in greenhouse up to 30 days from single node setts in portrays as sett treatment (@109 CFU ml-1 ). The promising isolate was characterized up to species level by PCR amplification of 16S rDNA region using Pseudomonas specific primers. Elite strain was identified as P. putida strain AKP-1.
9p
kethamoi4
16-04-2020
9
2
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China aster (Callistephus chinensis L. Nees.) is one of the important flower crop in India. It belongs to the family Asteraceae is native to China. Phyllody disease in China aster was first reported during 1986 from Bengaluru, India and known to be transmitted by Orosius albicinctus. The disease was characterized by chlorosis, upright growth, small leaf, short internode, stunting, profuse vegetative growth and phyllody (transformation of floral organs into leaf-like structures).
7p
caygaocaolon3
27-02-2020
19
1
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Marssonina leaf blotch caused by the fungus Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis causing premature leaf fall is one of the major economic constraint which hampers apple production in India. Seven isolates of Marssonina coronaria were isolated and evaluated for their morphological and molecular characterization using ITS primers. All the seven isolates were identified based on the morphological characteristics, and produced brown to black colonies without aerial mycelium, formed a wrinkled surface, on PDA after incubation for 30 days.
8p
chauchaungayxua3
07-02-2020
14
0
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The fungus Venturia carpophila causes scab in almond. In order to gain insight, an in vitro culture of the fungus has been established and its identity confirmed by its nr DNA. Internal transcribed spacer-ribosomal DNA (ITS-rDNA); the fungal molecular marker was used for molecular analysis. The target region of rDNA (ITS1-5.8S-ITS2) of this species was amplified using universal fungal primers (ITS1 and ITS4).
7p
chauchaungayxua3
07-02-2020
12
1
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