RNA decay
-
Ebook "mRNA decay: Methods and protocols" explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas.
231p lucchinguyen 28-12-2023 7 2 Download
-
Most eukaryotic genes are subject to alternative splicing (AS), which may contribute to the production of protein variants or to the regulation of gene expression via nonsense-mediated messenger RNA (mRNA) decay (NMD).
15p vialfrednobel 29-01-2022 8 0 Download
-
About 11% of all human genetic diseases are caused by nonsense mutations that generate premature translation termination codons (PTCs) in messenger RNAs (mRNA). PTCs not only lead to the production of truncated proteins, but also often result in decreased mRNA abundance due to nonsense-mediated mRNA decay (NMD).
16p vialfrednobel 29-01-2022 12 0 Download
-
RNA levels detected at steady state are the consequence of multiple dynamic processes within the cell. In addition to synthesis and decay, transcripts undergo processing. Metabolic tagging with a nucleotide analog is one way of determining the relative contributions of synthesis, decay and conversion processes globally.
17p viaristotle 29-01-2022 11 0 Download
-
Small nucleolar RNAs (snoRNAs) are mid-size non-coding RNAs required for ribosomal RNA modification, implying a ubiquitous tissue distribution linked to ribosome synthesis. However, increasing numbers of studies identify extraribosomal roles of snoRNAs in modulating gene expression, suggesting more complex snoRNA abundance patterns.
24p viarchimedes 26-01-2022 11 0 Download
-
Canonical nonsense-mediated decay (NMD) is an important splicingdependent process for mRNA surveillance in mammals. However, processed pseudogenes are not able to trigger NMD due to their lack of introns. It is largely unknown whether they have evolved other surveillance mechanisms.
21p viarchimedes 26-01-2022 6 0 Download
-
Alternative splicing, which generates multiple mRNA isoforms from single genes, is crucial for the regulation of eukaryotic gene expression. The flux through competing splicing pathways cannot be determined by traditional RNA-Seq, however, because different mRNA isoforms can have widely differing decay rates.
21p viarchimedes 26-01-2022 13 0 Download
-
The genome-wide mRNA half-lives were determined by a dynamic analysis of transcriptomes after transcription arrest. We have combined the analysis of mRNA half-lives with the steady-state concentrations (transcriptome) to provide an integrated overview of the in vivo activity of these exoribonucleases at the genome-scale.
13p vitzuyu2711 29-09-2021 6 1 Download
-
Trait loss is a pervasive phenomenon in evolution, yet the underlying molecular causes have been identified in only a handful of cases. Most of these cases involve loss-of-function mutations in one or more traitspecific genes. However, in parasitoid insects the evolutionary loss of a metabolic trait is not associated with gene decay.
14p visilicon2711 20-08-2021 6 1 Download
-
Pumilio RNA-binding proteins are evolutionarily conserved throughout eukaryotes and are involved in RNA decay, transport, and translation repression in the cytoplasm. Although a majority of Pumilio proteins function in the cytoplasm, two nucleolar forms have been reported to have a function in rRNA processing in Arabidopsis.
13p vijichea2711 28-05-2021 8 1 Download
-
RNA-seq data is currently underutilized, in part because it is difficult to predict the functional impact of alternate transcription events. Recent software improvements in full-length transcript deconvolution prompted us to develop spliceR, an R package for classification of alternative splicing and prediction of coding potential.
7p vikentucky2711 26-11-2020 9 0 Download
-
Allele specific expression (ASE) has become an important phenotype, being utilized for the detection of cis-regulatory variation, nonsense mediated decay and imprinting in the personal genome, and has been used to both identify disease loci and consider the penetrance of damaging alleles.
10p vioklahoma2711 19-11-2020 4 0 Download
-
The humanSUV3gene encodes an NTP-dependent DNA⁄RNA DExH box helicase predominantly localized in mitochondria. Its orthologue in yeast is a component of the mitochondrial degradosome complex involved in the mtRNA decay pathway. In contrast to this, the physiological func-tion of human SUV3remains to be elucidated. In this report we demon-strate that the hSuv3 protein interacts with HBXIP, previously identified as a cofactor of survivin in suppression of apoptosis and as a protein that binds the HBx protein encoded by the hepatitis B virus....
12p fptmusic 12-04-2013 36 1 Download
-
Because P2P systems designers have to a large extent failed to overcome these challenges, the semantics provided by these systems is typically quite weak. In most content sharing systems, only popular content is readily accessible — yet content popularity seems to be driven by Zipf distributions, in which a large fraction of requests are directed to unpopular content. Similarly, current content sharing systems ignore problems such as updates to content, and they typically only support retrieval of objects by name.
5p yasuyidol 02-04-2013 44 5 Download
-
The RNA degradosome is built on the C-terminal half of ribonuclease E (RNase E) which shows high sequence variation, even amongst closely related species. This is intriguing given its central role in RNA processing and mRNA decay. Previously, we have identified RhlB (ATP-dependent DEAD-box RNA helicase)-binding
13p viettel02 19-02-2013 25 1 Download
-
The efficient turnover of messenger RNA represents an important mecha-nism that allows the cell to control gene expression. Until recently, the mechanism of mRNA decay was mainly attributed to exonucleases, com-prising enzymes that degrade RNAs from the ends of the molecules.
15p mobifone23 18-01-2013 48 4 Download
-
RNA turnover and processing have now been demonstrated to be important steps that directly affect protein synthesis and the cell’s ability to survive in nature. However, the analysis of mRNA decay and polyadenylation in Escherichia coli has long been considered technically difficult. The development over the past 15 years of methods for the isolation and characterization of both mRNA and polyadenylated species has made the study of these important pathways of RNA metabolism more straightforward (Arraiano et al., 1988; O’Hara et al., 1995; Mohanty and Kushner, 1999)....
584p maket1311 16-10-2012 74 13 Download