Sequence optimization

Evaluation of human epidermal growth factor receptor 2 (HER2) overexpression caused by erbb2 receptor tyrosine kinase 2 (ERBB2) amplification (AMP) by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) is essential for treating unresectable metastatic gastric cancer (GC).

13p vikoch 27-06-2024 1 1   Download

Ebook "Introduction to computational biology: An evolutionary approach" Analysis of molecular sequence data is the main subject of this introduction to computational biology. There are two closely connected aspects to biological sequences: (i) their relative position in the space of all other sequences, and (ii) their movement through this sequence space in evolutionary time. Accordingly, the first part of the book deals with classical methods of sequence analysis: pairwise alignment, exact string matching, multiple alignment, and hidden Markov models.

329p tracanhphuonghoa1007 22-04-2024 10 2   Download

Targeted therapies specifically act by blocking the activity of proteins that are encoded by genes critical for tumorigenesis. However, most cancers acquire resistance and long-term disease remission is rarely observed. Understanding the time course of molecular changes responsible for the development of acquired resistance could enable optimization of patients’ treatment options.

22p vibransone 28-03-2024 3 2   Download

Intertumoral heterogeneity represents a significant hurdle to identifying optimized targeted therapies in gastric cancer (GC). To realize precision medicine for GC patients, an actionable gene alteration-based molecular classification that directly associates GCs with targeted therapies is needed.

12p vioraclene 31-03-2024 5 2   Download

Given the rarity of most single-gene Mendelian disorders, concerted efforts of data exchange between clinical and scientific communities are critical to optimize molecular diagnosis and novel disease gene discovery.

15p vioraclene 31-03-2024 2 2   Download

Here, we describe a novel approach for rapid discovery of a set of tumor-specific genomic structural variants (SVs), based on a combination of low coverage cancer genome sequencing using Oxford Nanopore with an SV calling and filtering pipeline. We applied the method to tumor samples of high-grade ovarian and prostate cancer patients and validated on average ten somatic SVs per patient with breakpoint-spanning PCR mini-amplicons.

14p vibransone 28-03-2024 4 2   Download

Part 1 book "Chemical process design and integration" includes content: The nature of chemical process design and integration, process economics, optimization, chemical reactors I – reactor performance, chemical reactors II – reactor conditions, chemical reactors III – reactor configuration, separation of homogeneous fluid mixtures I – distillation, separation of homogeneous fluid mixtures II – other methods, distillation sequencing.

272p muasambanhan08 01-03-2024 4 1   Download

Part 2 book "Chemical process design and integration" includes content: Distillation sequencing for azeotropic distillation, heat exchange, pumping and compression, continuous process recycle structure, heat exchanger networks I – network targets, continuous process simulation and optimization, heat exchanger networks II – network design, batch processes, heat exchanger networks III – stream data, heat integration of reactors.

316p muasambanhan08 01-03-2024 2 1   Download

The precise characterization of individual tumors and immune microenvironments using transcriptome sequencing has provided a great opportunity for successful personalized cancer treatment.

21p vicwell 29-02-2024 3 2   Download

Ebook "RNA detection: Methods and protocols" introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA;...

492p lucchinguyen 28-12-2023 6 2   Download

Ebook "Synthetic metabolic pathways: Methods and protocols" outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering.

352p lucchinguyen 28-12-2023 7 2   Download

Part 1 book "Diagnostic MRI in dogs and cats" includes content: General principles of magnetic resonance imaging, image characteristics in MRI and principal pulse sequences, MRI artifacts, optimized technique - brain, congenital and developmental disorders, MRI of non neurological structures of the head and neck.

423p oursky08 01-11-2023 2 1   Download

Molecular subtypes predict prognosis in muscle-invasive bladder cancer (MIBC) and are explored as predictive markers. To provide a common base for molecular subtyping and facilitate clinical applications, a consensus classification has been developed.

10p visharma 20-10-2023 3 2   Download

The authors intend to compare the effects of each targeted therapy (TT) in the treatment of patients with metastatic renal cell carcinoma (mRCC) using big data based on the Korean National Health Insurance System (NHIS) and determine the optimal treatment sequence.

13p visharma 20-10-2023 5 2   Download

Part 1 book "Principles of sequencing and scheduling" includes content: Introduction, single machine sequencing, optimization methods for the single machine problem, heuristic methods for the single machine problem, earliness and tardiness costs, sequencing for stochastic scheduling, safe scheduling, extensions of the basic model, parallel machine models.

292p oursky03 21-08-2023 7 5   Download

Part 1 book "Principles of sequencing and scheduling" includes content: Introduction, single-Machine sequencing, optimization methods for the single - machine problem, heuristic methods for the single -machine problem, earliness and tardiness costs, sequencing for stochastic scheduling, safe scheduling, extensions of the basic model.

215p oursky01 24-07-2023 7 3   Download

Androgen insensitivity syndrome (AIS) is a rare X-linked recessive androgen receptor (AR) disorder. However, the overlap in clinical manifestations between AIS and other disorders of sex development can cause clinical diagnostic difficulties. Applying the whole coding region sequencing method is an optimal method for the diagnosis of AIS.

8p vicaptainmarvel 21-04-2023 3 2   Download

In a previous study, a gene GL0694641 coding for endoglucanase containing 3 domains GH5- CBM72-CBM72 was exploited from metagenomic DNA data of bacteria in Vietnamese goats’ rumen. The gene (eg5) encoding the mature enzyme (without signal peptide coding sequence) was optimized codons, artificially synthesized, and inserted into the pET22b(+) vector at NcoI and XhoI to generate expression vector pET22-eg5 for expression of the gene in Eschrichia coli.

11p vicaptainmarvel 21-04-2023 5 2   Download

Advanced Algorithms Analysis and Design - Lecture 23: Longest common subsequence (Dynamic algorithm) & Optimal binary search trees. In this lecture we will cover the following: optimal substructure of an LCS; computable tables; longest common subsequence algorithm; construction of longest common subsequence; relationship with shortest common supper-sequence;...

33p andromedashun 26-05-2022 4 1   Download

CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dCas9/gRNA genome labeling systems.

10p vimichaelfaraday 25-03-2022 23 1   Download