Sequencing and in silico characterization

Ebook "Circular RNAs: Methods and protocols" provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

223p lucchinguyen 28-12-2023 3 2   Download

Genome-wide data are invaluable to characterize differentiation and adaptation of natural populations. Reduced representation sequencing (RRS) subsamples a genome repeatedly across many individuals. However, RRS requires careful optimization and fine-tuning to deliver high marker density while being cost-efficient.

20p vitzuyu2711 29-09-2021 7 2   Download

Keeping in view above considerations, the present study was framed for isolation, biochemical characterization and molecular identification of iron nanoparticles synthesizing bacteria by use of 16S rRNA technology to determine evolutionary rates of different bacterial isolates and close synteny between them by using various bioinformatics tools.

12p angicungduoc8 07-11-2020 26 3   Download

Glycogen storage disease type Ia (GSD Ia), a rare autosomal inherited disorder, is characterized by accumulation of excessive glycogen and fat in the liver. Primary symptoms of GSD Ia include hypoglycemia; metabolic acidosis; elevated levels of lactate, uric acid and lipids; hepatomagaly and growth retardation. Glycogen storage disease type Ia was caused by mutations in the G6PC gene. In this study, mutations in a Vietnamese patient with glycogen storage disease type Ia were analyzed using the whole exome sequencing method. A missense mutation c.356A>T (p.

8p xilank_of 22-07-2020 13 1   Download

MicroRNAs (miRNAs) are highly conserved class of short endogenous non-coding small RNA molecules of about 18–22 nucleotide in length. MicroRNAs negatively regulate the gene expression by degrading target mRNA. In the present study, comparative genomic based approach was used to identify and characterize new conserved microRNAs in “orphan legume crop” pigeon pea (Cajanus cajan L.) using expressed sequence tags (ESTs) by in silico method. A total of 4,621 unique previously reported microRNAs were used for homology search in 25,576 ESTs of pigeon pea for identifying conserved miRNAs.

11p nguaconbaynhay6 24-06-2020 12 2   Download

Salmonella is a major foodborne bacterial pathogen responsible for millions of cases of infection and thousands of death in humans across the globe annually. Poultry and poultry products are considered to be the major source of Salmonella infection in humans. The economic loss caused by salmonellosis is very huge in case of poultry industry. Vaccination of the poultry is found to efficiently reduce the zoonotic risks associated with Salmonella infection.

11p angicungduoc4 26-04-2020 9 0   Download

Tomato leaf curl virus disease (ToLCVD) is one of the major causes for highest economical loss of tomato in India. In this study, Natural incidence of ToLCV disease on tomato was observed at Gujarat, India in the March, 2019. The infected plants revealed systemic viral symptoms in the form of sever leaf curling, leaf crumple with marginal yellowing, stem warped and stunted. Molecular methods were followed to further characterized coat protein (CP) gene of tomato leaf curl virus using specified primers.

11p trinhthamhodang3 12-02-2020 13 1   Download

Mung bean is a widely consumed tropical food grain legume and is generally affected by different abiotic stresses. Late Embryogenic Abundant (LEA) proteins help the plants to withstand these stresses by its multi-functional role. In this study, a VrLEA2 gene isolated from mung bean using gene specific primers was sequenced and the in silico analysis of the gene sequence revealed the presence of eight ORF regions. ORF6region showed high similarity to Em like protein of Vigna radiata (L) Wilczek.

8p chauchaungayxua3 07-02-2020 16 1   Download

Minor millets are under-exploited plant species often dubbed as ‘Crops for the future’ as they are best adapted to less fertile area and can survive limited water situations and relatively free from pests and diseases. Degenerate oligo-PCR strategy was employed to isolate resistant gene analogues from minor millets by amplifying samples using NBSLRR gene specific primers. Genomic DNA was isolated from the young leaves of minor millets viz., Foxtail millet (Setaria italica) var. CO 5 and Finger millet (Eleusine corocana) var. CO (Ra) 14 by following CTAB method with minor modifications.

9p nguaconbaynhay1 04-12-2019 12 0   Download

16S rRNA gene sequences are most commonly used for determination or studying the bacterial phylogeny and taxonomy because its present in almost all bacterial population. 16S rRNA gene sequence is remaining conversed during evolution so that 16S rRNA gene identification method is widely used for identification of bacterial diversity. In this study, bacterial population was isolated from soil sample for copper nanoparticles synthesis and maximum copper synthesizing bacteria further used for characterization.

6p nguaconbaynhay1 04-12-2019 27 1   Download