Single samples
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The 3D printing PMMA/ZrO2 filaments with a diameter of 1.75±0.05 mm were fabricated by using a single-screw extruder at temperatures of four heating zones of 190, 200, 210, and 210°C. Testing samples were prepared from the extruded filaments by using a fusion deposition modeling 3D printer and a Haake MiniJet piston injection molding machine. Fourier transform infrared spectroscopy, mechanical properties, field emission scanning electron microscopy (FESEM), differential scanning calorimetry, and thermogravimetric analysis of the filaments were investigated.
9p dianmotminh02 03-05-2024 1 1 Download
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We developed subclone multiplicity allocation and somatic heterogeneity (SMASH), a new statistical method for intra-tumor heterogeneity (ITH) inference. SMASH is tailored to the purpose of large-scale association studies with one tumor sample per patient. In a pan-cancer study of 14 cancer types, we studied the associations between survival time and ITH quantified by SMASH, together with other features of somatic mutations.
15p vibransone 28-03-2024 7 2 Download
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Mosaic mutations acquired during early embryogenesis can lead to severe early-onset genetic disorders and cancer predisposition, but are often undetectable in blood samples. The rate and mutational spectrum of embryonic mosaic mutations (EMMs) have only been studied in few tissues, and their contribution to genetic disorders is unknown.
14p vibransone 28-03-2024 2 2 Download
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Populations of closely related microbial strains can be simultaneously present in bacterial communities such as the human gut microbiome. We recently developed a de novo genome assembly approach that uses read cloud sequencing to provide more complete microbial genome drafts, enabling precise differentiation and tracking of strain-level dynamics across metagenomic samples.
17p vibransone 28-03-2024 9 1 Download
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When interpreting sequencing data from multiple spatial or longitudinal biopsies, detecting sample mix-ups is essential, yet more difficult than in studies of germline variation. In most genomic studies of tumors, genetic variation is detected through pairwise comparisons of the tumor and a matched normal tissue from the sample donor.
9p vibransone 28-03-2024 2 2 Download
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A population-based gene expression signature of molecular clock phase from a single epidermal sample
For circadian medicine to influence health, such as when to take a drug or undergo a procedure, a biomarker of molecular clock phase is required––one that is easily measured and generalizable across a broad population. It is not clear that any circadian biomarker yet satisfies these criteria.
12p vibransone 28-03-2024 8 1 Download
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Genome-wide association studies (GWAS) have identified pervasive sharing of genetic architectures across multiple immune-mediated diseases (IMD). By learning the genetic basis of IMD risk from common diseases, this sharing can be exploited to enable analysis of less frequent IMD where, due to limited sample size, traditional GWAS techniques are challenging.
17p vibransone 28-03-2024 4 2 Download
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In this study we generated a whole exome sequencing benchmark dataset using the platinum genome sample NA12878 and developed an intersect-then-combine (ITC) approach to increase the accuracy in calling single nucleotide variants (SNVs) and indels in tumour-normal pairs. We evaluated the effect of alignment, base quality recalibration, mutation caller and filtering on sensitivity and false positive rate.
11p vioraclene 31-03-2024 4 2 Download
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Allele-specific expression (ASE) is differential expression of each of the two chromosomal alleles of an autosomal gene. We assessed ASE patterns in the human left atrium (LA, n = 62) and paired samples from the left ventricle (LV, n = 76) before and after ischemia, and tested the utility of differential ASE to identify genes associated with postoperative atrial fibrillation (poAF) and myocardial ischemia.
11p vioraclene 31-03-2024 3 2 Download
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Cryopreservation of human cancers conserves tumour heterogeneity for singlecell multi-omics analysis
High throughput single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool for exploring cellular heterogeneity among complex human cancers. scRNA-Seq studies using fresh human surgical tissue are logistically difficult, preclude histopathological triage of samples, and limit the ability to perform batch processing.
17p vibransone 28-03-2024 1 1 Download
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The benefit of precision medicine based on relatively limited gene sets and often-archived samples remains unproven. PERMED-01 (NCT02342158) was a prospective monocentric clinical trial assessing, in adults with advanced solid cancer, the feasibility and impact of extensive molecular profiling applied to newly biopsied tumor sample and based on targeted NGS (t-NGS) of the largest gene panel to date and whole-genome arraycomparative genomic hybridization (aCGH) with assessment of single-gene alterations and clinically relevant genomic scores.
20p vibransone 28-03-2024 3 1 Download
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Human pancreatic islets are a central focus of research in metabolic studies. Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing (scRNAseq). We introduce single-nucleus RNA-sequencing (snRNA-seq) as an alternative approach for investigating transplanted human islets.
17p vibransone 28-03-2024 4 2 Download
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Ebook "Consequences of combinatorial studies of positive electrodes for li-ion batteries" help resolve a number of points of confusion and contradiction in the literature. Amongst other important findings, the compositions and synthesis conditions giving rise to layered-layered nano-composites are presented and electrochemical results are used to show how better electrode materials can be achieved by making samples in the single phase-layered regions.
174p tudohanhtau1006 29-03-2024 2 1 Download
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Recent investigations of the meninges have highlighted the importance of the dura layer in central nervous system immune surveillance beyond a purely structural role. However, our understanding of the meninges largely stems from the use of pre-clinical models rather than human samples.
25p viellison 28-03-2024 3 1 Download
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Previous large-scale studies of de novo variants identified a number of genes associated with neurode‑ velopmental disorders (NDDs); however, it was also predicted that many NDD-associated genes await discovery. Such genes can be discovered by integrating copy number variants (CNVs), which have not been fully considered in previous studies, and increasing the sample size.
20p viellison 28-03-2024 3 2 Download
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Epigenetic characterization of cell-free DNA (cfDNA) is an emerging approach for detecting and characterizing diseases such as cancer. We developed a strategy using nanopore-based single-molecule sequencing to measure cfDNA methylomes. This approach generated up to 200 million reads for a single cfDNA sample from cancer patients, an order of magnitude improvement over existing nanopore sequencing methods.
13p vicwell 29-02-2024 2 1 Download
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Very low-coverage (0.1 to 1×) whole genome sequencing (WGS) has become a promising and aford‑ able approach to discover genomic variants of human populations for genome-wide association study (GWAS).
18p vicwell 29-02-2024 2 1 Download
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Part 1 book "Probability and statistics for engineering and the sciences" includes content: Overview and descriptive statistics; probability; discrete random variables and probability distributions; continuous random variables and probability distributions; joint probability distributions and random samples; point estimation; statistical intervals based on a single sample; tests of hypotheses based on a single sample.
378p muasambanhan06 01-02-2024 2 1 Download
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Part 1 book "Applied statistics and probability for engineers" includes content: The role of statistics in engineering; probability; discrete random variables and probability distributions; continuous random variables and probability distributions; joint probability distributions; descriptive statistics; sampling distributions and point estimation of parameters; statistical intervals for a single sample; tests of hypotheses for a single sample.
366p muasambanhan06 01-02-2024 7 1 Download
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Part 2 book "Applied statistics and probability for engineers" includes content: Statistical inference for two samples; simple linear regression and correlation; multiple linear regression; design and analysis of single factor experiments - the analysis of variance; design of experiments with several factors; statistical quality control.
424p muasambanhan06 01-02-2024 6 0 Download