Skeletal muscle

This study evaluated the feasibility and safety of non-intubated anesthesia VATS for patients with MG to solve this issue. It was a prospective, descriptive study involving six patients aged 18 to 60, ASA I, II, who underwent VATS thymectomy under non-intubated anesthesia and local anesthesia at Vietduc University Hospital between May 2022 and December 2023.

12p vinatisu 23-08-2024 1 1   Download

The prognostic value of a low skeletal mass index (SMI) has been investigated in locally advanced oesophageal (LAE) cancer at diagnosis. However, nothing is known about its evolution and clinical impact between initial diagnosis and recurrence.

10p vielonmusk 21-01-2022 8 1   Download

Sarcopenia is defined by a loss of muscle strength associated to a decrease in skeletal muscle mass. Ageing greatly contributes to sarcopenia as may many other factors such as cancer or androgen deprivation thera‑ pies (ADT).

7p vielonmusk 21-01-2022 13 1   Download

In the present study, we use LPS to treat C2C12 skeletal muscle cells and observe expression of several inflammatory makers. Our results show that expression of inflammatory cytokine IL6 mRNA is strongly induced in the LPS-treated C2C12 cells compared to the control cells. Surprisingly, expression of the upper controller of inflammation TLR2 mRNA in the LPS-treated C2C12 cells is similar as that in the control cells.

6p koxih_kothogmih5 04-09-2020 11 1   Download

Chapter 9 - Muscles and muscle tissue (part a) provides knowledge of muscle tissues and skeletal muscle. The following will be discussed in this chapter: Types of muscle tissue, special characteristics of muscle tissue, muscle functions, gross anatomy of a skeletal muscle, microscopic anatomy of a skeletal muscle fiber, sliding filament model of contraction, physiology of skeletal muscle fibers,...

65p tangtuy07 02-04-2016 59 3   Download

This chapter presents the following content: Gross anatomy of a skeletal muscle, microscopic anatomy of a skeletal muscle fiber, sliding filament model of contraction, physiology of skeletal muscle fibers, contraction of a skeletal muscle, muscle metabolism, force of muscle contraction, velocity and duration of contraction, adaptations to exercise.

42p tangtuy07 02-04-2016 54 3   Download

The muscular system (part a), the following will be discussed in this chapter: Skeletal muscles: functional groups, naming skeletal muscles, muscle mechanics: arrangement of fascicles, muscle mechanics: lever systems, classes of lever systems,...

48p tangtuy07 02-04-2016 47 3   Download

Aralar1 and citrin are members of the subfamily of calciumbinding mitochondrial carriers and correspond to two isoforms of the mitochondrial aspartate/glutamate carrier (AGC). These proteins are activated by Ca2+ acting on the external side of the inner mitochondrial membrane. Although it is known that aralar1 is expressed mainly in skeletal muscle, heart and brain, whereas citrin is present in liver, kidney and heart, the precise tissue distribution of the two proteins in embryonic and adult tissues is largely unknown....

8p system191 01-06-2013 34 4   Download

Recently, we have identified and purified minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.

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Uncoupling protein-3 (UCP3) is a mitochondrial innermembrane protein abundantly expressed in rodent and human skeletal muscle which may be involved in energy dissipation. Many studies have been performed on the metabolic regulation of UCP3 mRNA level, but little is known about UCP3 expression at the protein level. Two populations of mitochondria have been described in skeletal muscle, subsarcolemmal (SS) and intermyofibrillar (IMF), which differ in their intracellular localization and possibly also their metabolic role....

7p system191 01-06-2013 35 4   Download

Mitochondrial DNA (mtDNA) deletion mutations accumulate with age in tissues of a variety of species. Although the relatively low calculated abundance of these deletion mutations in whole tissue homogenates led some investigators to suggest that these mutations do not have any physiological impact, their focal and segmental accumulation suggests that they can, and do, accumulate to levels sufficient to affect the metabolism of a tissue.

6p research12 01-06-2013 23 4   Download

We have previously demonstrated that the steroid hormone 1a,25(OH)2-vitamin D3 [1a,25(OH)2D3] stimulates the production ofinositol trisphosphate (InsP3), the breakdown product ofphosphatidylinositol 4,5-biphosphate (PtdInsP2) by phospholipase C (PtdIns-PLC), and activates the cyto-solic tyrosine kinase c-Src in skeletal muscle cells. In the present study we examined whether 1a,25(OH)2D3induces the phosphorylation and membrane translocation ofPLCc and the mechanism involved in this isozyme activation. ...

10p research12 29-04-2013 44 4   Download

The uncoupling proteins (UCPs) are thought to uncouple oxidative phosphorylation in the mitochondria and thus generate heat. One of the UCP isoforms, UCP3, is abun-dantly expressed in skeletal muscle, the major thermogenic tissue in humans. UCP3 has been overexpressed at high levels inyeast systems,where it leads to theuncouplingof cell respiration, suggesting that UCP3may indeed be capable of dissipating the mitochondrial proton gradient.

9p research12 29-04-2013 37 3   Download

Ikkai&Ooi [Ikkai,T.&Ooi,T. (1966)Biochemistry5, 1551± 1560] made a thorough study of the e€ect of pressure on G- and F-actins. However, all of the measurements in their study were made after the release of pressure. In the present experiment in situ observations were attempted by using eATP to obtain further detailed kinetic and thermodynamic information about the behaviour of actin under pressure.

8p research12 29-04-2013 41 7   Download

Vertebrate troponin regulates muscle contraction through alternative bind-ing of the C-terminal region of the inhibitory subunit, troponin-I (TnI), to actin or troponin-C (TnC) in a Ca 2+ -dependent manner. To elucidate the molecular mechanisms of this regulation by molluskan troponin, we com-pared the functional properties of the recombinant fragments of Akazara scallop TnI and rabbit fast skeletal TnI.

12p fptmusic 11-04-2013 31 2   Download

Calpain 1, a ubiquitous calcium-dependent intracellular protease, was recently found in a tight association with myofibrils in skeletal muscle tis-sue [Delgado EF, Geesink GH, Marchello JA, Goll DE & Koohmaraie M (2001) J Anim Sci79, 2097–2107). Our immunofluorescence and immuno-electron microscopy investigations restrain the protease location at the per-iphery of the Z-band and at the midpoint of the I-band.

13p awards 06-04-2013 35 4   Download

The troponin (Tn) complex is formed by TnC, TnI and TnT and is respon-sible for the calcium-dependent inhibition of muscle contraction. TnC and TnI interact in an antiparallel fashion in which the N domain of TnC binds in a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin interaction. While the crys-tal structure of the core cardiac muscle troponin complex has been deter-mined, very little high resolution information is available regarding the skeletal muscle TnI–TnC complex. ...

0p awards 05-04-2013 34 3   Download

The effect of gadolinium ions on the sarcoplasmic reticulum (SR) calcium release channel⁄ryanodine receptor (RyR1) was studied using heavy SR (HSR) vesicles and RyR1 isolated from rabbit fast twitch muscle. In the [ 3 H]ryanodine binding assay, 5lmGd 3+ increased the Kd of the [ 3 H]ry-anodine binding of the vesicles from 33.8 nmto 45.6 nmwhileBmax, refer-ring to the binding capacity, was not affected significantly. In the presence of 18 nm[ 3 H]ryanodine and 100lmfree Ca 2+ ,Gd 3+ inhibited the binding of the radiolabeled ryanodine with an apparentKd value of 14.

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Titin is known to interact with actin thin filaments within the I-band region of striated muscle sarcomeres. In this study, we have used a titin fragment of 800 kDa (T800) purified from striated skeletal muscle to measure the effect of this interaction on the functional properties of the actin– myosin complex. MALDI-TOF MS revealed that T800 contains the entire titin PEVK (Pro, Glu, Val, Lys-rich) 1 domain.

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A naturally occurring variant of the human androgen receptor (AR) named AR45 has been identified. It lacks the entire region encoded by exon 1 of the ARgene and is composed of the AR DNA-binding domain, hinge region and ligand-binding domain, preceded by a novel seven amino-acid long N-terminal extension. A survey of human tissues revealed that AR45 was expressed mainly in heart and skeletal muscle. In cotransfection experiments, AR45 inhibited AR function, an effect necessitating intact DNA- and ligand-binding properties. ...

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