Spacer region of rDNA

In order to accurately identify ginseng species (Panax spp.) that grow naturally in some Northern provinces of Vietnam, we collected 30 natural ginseng samples and used nuclear genetic region (ITSrDNA) for current analysis.

9p vimulcahy 18-09-2023 4 3   Download

Inocybe viscida sp. nov., a novel species found in a forest with old trees of Pinus brutia and tertiary relict endemic Liquidambar orientalis in coastal area of southwestern Turkey, is described and illustrated. We present this new species based on morphological and molecular data from nuclear rDNA internal transcribed spacer region (ITS1-5.8SITS2 = ITS) and the portions of nuc 28S rRNA (LSU) gene. Comprehensive description, field photographs, line drawings of the microscopic features and comparisons with phenetically similar taxa and phylogenetically related species are discussed.

11p lyhuyenthu 31-01-2023 9 2   Download

The whole repeat unit of 5S rDNA and the internal transcribed spacer (ITS) of four European Donax species were analysed. After amplifying, cloning and sequencing several 5S and ITS units, their basic features and their variation were described.

13p vihagrid 30-01-2023 11 3   Download

Pluteus and Volvopluteus are two closely related genera of Pluteaceae that comprise saprotrophic fungi. Although the genera have been well-studied in Europe and North and South America, the diversity of Pluteus, mainly Pluteus sect. Pluteus, and Volvopluteus in Turkey is poorly known due to insufficient morphological descriptions and a lack of DNA sequence data. In this study, the diversity of Pluteus sect. Pluteus and Volvopluteus in Turkey is evaluated by employing both morphological features and phylogenetic approaches. A general phylogeny of Pluteus sect.

19p tudichquannguyet 29-11-2021 8 1   Download

A molecular key based on the sequence of the internal transcribed spacer (ITS) of the ribosomal cistron was used for the identification of common Trichogramma (Hymenoptera: Trichogramma tidae) species found in agricultural field of Alhassa oasis in Saudi Arabia. The aligned sequences were checked using BLAST program at GenBank. The sequences of the three ITS region were the same and shared identities ranged from 98.3 to 99.4% against all Trichogramma cacoeciae sequences at the GenBank.

8p quenchua6 15-06-2020 13 2   Download

Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through ITS1-5.8s-ITS 4 primer series.

10p trinhthamhodang1213 30-05-2020 8 0   Download

The fungus Venturia carpophila causes scab in almond. In order to gain insight, an in vitro culture of the fungus has been established and its identity confirmed by its nr DNA. Internal transcribed spacer-ribosomal DNA (ITS-rDNA); the fungal molecular marker was used for molecular analysis. The target region of rDNA (ITS1-5.8S-ITS2) of this species was amplified using universal fungal primers (ITS1 and ITS4).

7p chauchaungayxua3 07-02-2020 12 1   Download

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates.

8p chauchaungayxua3 07-02-2020 33 0   Download

A new species of the genus Barssia was identified from Turkey using both morphological and phylogenetic analysis of the ribosomal internal transcribed spacer region (ITS rDNA) and nuclear ribosomal large subunit (LSU rDNA) gene sequences.

8p caplock2711 21-02-2019 27 1   Download

Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR).

5p aquaman27 15-01-2019 16 1   Download

The genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis.

11p vimb123 12-01-2019 24 1   Download