Transgenic Arabidopsis plant
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Owing to their sessile nature, plants are easily affected by various extenal factors. Among those, drought and salinity are considered as the most common stresses, which often pose a threat to plant growth and development. Major effects of the drought and salinity are interconnected and drive similar series of molecular changes in plants.
8p vimulcahy 18-09-2023 3 3 Download
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Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidopsis.
10p vihagrid 30-01-2023 6 3 Download
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It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses. This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the droughtstress responses in Caragana korshinskii.
10p vihagrid 30-01-2023 12 3 Download
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Hypoxia induced by flooding causes significant losses to crop production almost every year. However, the molecular network of submergence signaling pathway is still poorly understood. According to previous studies, transgenic plants overexpressing the WRKY33 gene showed enhanced resistance to submergence stress.
10p vihagrid 30-01-2023 5 3 Download
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The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis).
7p vigalileogalilei 27-02-2022 28 1 Download
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The SnRK2s (Plant specific protein kinase) are involved in various biological processes, such as plant defense and environmental challenges. In Arabidopsis, AtSnRK2s regulate the expression of some metal transporters.
13p visilicon2711 20-08-2021 5 0 Download
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As global warming becomes increasingly severe, it is urgent that we enhance the heat tolerance of crops. We previously reported that Arabidopsis thaliana PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C9 (AtPLC9) promotes heat tolerance.
11p vijichea2711 28-05-2021 9 1 Download
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The NAC (NAM, ATAF1/ATAF2, and CUC2) transcription factors belong to a large family of plantspecific transcription factors in monocot and dicot species. These transcription factors regulate the expression of stress tolerance-related genes that protect plants from various abiotic stresses, including drought, salinity, and low temperatures.
11p vijichea2711 28-05-2021 11 1 Download
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Drought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression.
19p vijichea2711 28-05-2021 8 1 Download
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The mitogen-activated protein kinases (MAPKs), as a part of the MAPKKK-MAPKK-MAPK cascade, play crucial roles in plant development as an intracellular signal transduction pathway to respond various environmental signals. However, few MAPKK have been functionally characterized in grapevine.
15p vijichea2711 28-05-2021 11 1 Download
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Sheepgrass (Leymus chinensis (Trin.) Tzvel) is a perennial forage grass that can survive extreme freezing winters (− 47.5 °C) in China. In this study, we isolated an unknown function MYB transcription factor gene, LcMYB4, from sheepgrass. However, the function of LcMYB4 and its homologous genes has not been studied in other plants.
15p vijichea2711 28-05-2021 6 1 Download
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Drought stress can severely affect plant growth and crop yield. The cloning and identification of drought-inducible promoters would be of value for genetically-based strategies to improve resistance of crops to drought.
14p vijichea2711 28-05-2021 7 1 Download
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Studies on soybean GmNAC085 transcription factor revealed that the gene expression in plants was induced by water shortage treatments and its overexpression in the model plant Arabidopsis displayed improved plant tolerance characteristics towards drought stress. In this study, we continued analyzing the biological functions of GmNAC085 using transgenic soybean system overexpressing GmNAC085 gene, by targeting at a number of plant physiological features and biochemical activities in response to limited water growing condition.
8p nguyenxuankha_bevandan 14-08-2020 26 3 Download
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Recently we showed that de novo expression of a turtle riboflavin-binding protein (RfBP) in transgenic Arabidopsis increased H2O2 concentrations inside leaf cells, enhanced the expression of floral regulatory gene FD and floral meristem identity gene AP1 at the shoot apex, and induced early flowering.
16p viminato2711 22-05-2020 12 2 Download
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Drought is by far the most important environmental factor contributing to yield losses in crops, including soybeans [Glycine max (L.) Merr.]. To address this problem, a gene that encodes an osmotin-like protein isolated from Solanum nigrum var. americanum (SnOLP) driven by the UBQ3 promoter from Arabidopsis thaliana was transferred into the soybean genome by particle bombardment.
9p viminato2711 22-05-2020 16 0 Download
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The novel mutant allele hsi2-4 was isolated in a genetic screen to identify Arabidopsis mutants with constitutively elevated expression of a glutathione S-transferase F8::luciferase (GSTF8::LUC) reporter gene in Arabidopsis.
19p viminato2711 22-05-2020 11 0 Download
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Promoters play important roles in gene expression and function. There are three basic types of promoters: Constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers.
13p viminato2711 22-05-2020 12 1 Download
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Extensive studies using the model system Arabidopsis thaliana to elucidate plant defense signaling and pathway networks indicate that salicylic acid (SA) is the key hormone triggering the plant defense response against biotrophic and hemi-biotrophic pathogens, while jasmonic acid (JA) and derivatives are critical to the defense response against necrotrophic pathogens.
19p viminato2711 22-05-2020 17 1 Download
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The pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses.
13p viminato2711 22-05-2020 13 0 Download
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Plants have evolved light sensing mechanisms to optimally adapt their growth and development to the ambient light environment. The COP1/SPA complex is a key negative regulator of light signaling in the well-studied dicot Arabidopsis thaliana.
15p viminato2711 22-05-2020 36 0 Download