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Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0× 107 cells at passage 2 (P2).
16p vidarwin 22-02-2022 16 1 Download
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We present RaGOO, a reference-guided contig ordering and orienting tool that leverages the speed and sensitivity of Minimap2 to accurately achieve chromosome-scale assemblies in minutes. After the pseudomolecules are constructed, RaGOO identifies structural variants, including those spanning sequencing gaps.
17p vielonmusk 30-01-2022 3 0 Download
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The assembly of large, repeat-rich eukaryotic genomes represents a significant challenge in genomics. While long-read technologies have made the high-quality assembly of small, microbial genomes increasingly feasible, data generation can be expensive for larger genomes. OPERA-LG is a scalable, exact algorithm for the scaffold assembly of large, repeatrich genomes, out-performing state-of-the-art programs for scaffold correctness and contiguity.
16p viaristotle 29-01-2022 11 0 Download
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We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing.
15p viarchimedes 26-01-2022 10 0 Download
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Continuous developments in DNA sequencing technologies aim at alleviating the technical challenges that limit the ability to assemble sequence data into full-length chromosomes. Conventional assembly programs and pipelines often encounter difficulties to close gaps in draft genome assemblies introduced by regions enriched in repeated elements.
22p viarchimedes 26-01-2022 14 0 Download
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Scaffold là chất mang hay còn gọi là giá đỡ sinh học, hay giàn giáo sinh học, có vai trò quan trọng trong công nghệ cấy ghép tế bào gốc. Bài này xin giới sơ lược về scaffold, chất liệu, cấu trúc, các đặc tính của scaffold và cách tạo hình 3D scaffold.
4p viericschmid 13-01-2022 11 0 Download
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Four 6-aminopenicillanic acid moieties were grafted at either rim of calix[4]arene, giving 2 novel generations of penicillin, which were named calixpenam. Antibiotic tests showed that they have amplified activity with respect to the corresponding penams against 3 gram-positive nonpenicillinase-producing strains of Streptococcus.
9p langthannam 29-12-2021 13 0 Download
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Wound dressings are one of the most rapidly expanding areas in medical textiles. In recent years, a large number of functional wound dressing has been developed. The aims of these products are to speed up the wound healing process and to provide maximum comfort for patients. Today, the multidisciplinary studies are required to improve further existed wound dressings.
10p langthannam 29-12-2021 8 0 Download
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The biocomposite demonstrated upgraded swelling and antibacterial activity. The addition of ZnHAP accomplished the antibacterial activity. Biocompatibility studies were carried out using osteoblast cells and thus we proved the non-toxic nature of the biocomposite. Osteoblast cell adhesion investigations demonstrated that the cells were appended and infiltrated into the inside of the biocomposite.
5p meyerowitz 25-12-2021 4 0 Download
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The current study adopted sociocultural discourse analysis framework to analyze scaffolding in verbal interaction in EFL university classes. The students were learning English from a prescribed textbook. Recorded talks of these students and their teachers in their natural classroom settings were sequenced to explore the role of scaffolding.
10p viwilliamleiding 04-12-2021 20 5 Download
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Cancer is one of the main global health problems. In order to develop novel antitumor agents, we synthesized 3,4-dihydropyrimidine-2(1H)-one (DHPM) and 2,6-diaryl-substituted pyridine derivatives as potential antitumor structures and evaluated their cytotoxic effects against several cancer cell lines.
20p tudichquannguyet 29-11-2021 7 1 Download
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Artificially fabricated hydroxyapatite (HAP) shows excellent biocompatibility with various kinds of cells and tissues which makes it an ideal candidate for a bone substitute material. In this study, hydroxyapatite nanoparticles have been prepared by using the wet chemical precipitation method using calcium nitrate tetra-hydrate [Ca(NO3)2.4H2O] and di-ammonium hydrogen phosphate [(NH4)2HPO4] as precursors.
17p tudichquannguyet 29-11-2021 8 1 Download
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Polyethylene glycol (PEG) is a polymer that is widely used as a carrier for drug delivery systems (DDS). A library of N-PEGylated quinoline derivatives of PEG molecular weight 200 was prepared rapidly after the activation of PEGs using maleic anhydride. Quinoline with a polymer backbone is essential as new material. PEG is a water-soluble nonionic polymer approved by food and drug organizations for medicine applications.
20p tudichquannguyet 29-11-2021 11 1 Download
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In this study, a fibrous bone tissue engineering scaffold was produced using poly(lactic-co-glycolic acid) (PLGA). Different concentrations (20%, 25%, and 30%) of PLGA (PLA:PGA 75:25) (Mw = 66,000–107,000) were wet spun using coagulation baths composed of different ratios (75:25, 60:40, 50:50) of isopropanol and distilled water. Scanning electron microscopy (SEM) and in vitro degradation studies were performed to characterize the fibrous PLGA scaffolds.
11p thiencuuchu 27-11-2021 4 1 Download
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In this study, a hydroxyapatite (HA) based scaffold was used together with human adipose stem cells (hASCs), human bone marrow stem cells (hBMSCs) and gingival epithelial cells to mimic human tooth dentin-pulp-enamel tissue complexes and model an immature tooth at the late bell stage in vitro. Characteristics of the scaffold were determined via SEM, FTIR, pore size and density measurements.
13p thiencuuchu 27-11-2021 9 1 Download
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Although single molecule sequencing is still improving, the lengths of the generated sequences are inevitably an advantage in genome assembly. Prior work that utilizes long reads to conduct genome assembly has mostly focused on correcting sequencing errors and improving contiguity of de novo assemblies.
9p vilarryellison 29-10-2021 4 1 Download
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Genomes rearrangements carry valuable information for phylogenetic inference or the elucidation of molecular mechanisms of adaptation. However, the detection of genome rearrangements is often hampered by current deficiencies in data and methods: Genomes obtained from short sequence reads have generally very fragmented assemblies, and comparing multiple gene orders generally leads to computationally intractable algorithmic questions.
15p vibeauty 23-10-2021 11 0 Download
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Obtaining complete gene structures is one major goal of genome assembly. Some gene regions are fragmented in low quality and high-quality assemblies. Therefore, new approaches are needed to recover gene regions. Genomes are widely transcribed, generating messenger and non-coding RNAs. These widespread transcripts can be used to scaffold genomes and complete transcribed regions.
13p vibeauty 23-10-2021 8 0 Download
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The ability to generate long sequencing reads and access long-range linkage information is revolutionizing the quality and completeness of genome assemblies. Here we use a hybrid approach that combines data from four genome sequencing and mapping technologies to generate a new genome assembly of the honeybee Apis mellifera.
19p visilicon2711 20-08-2021 9 1 Download
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The behavior of cells in vivo is complex and highly dynamic, as it results from an interplay between intercellular matrix proteins with surface receptors and other microenvironmental cues.
15p vilichoo2711 23-06-2021 6 1 Download
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