Analysis of sera from cattle suspected for brucellosis by agglutination tests and ELISA

Brucellosis is a major bacterial zoonosis of global importance. Bovine brucellosis is endemic in all states of India. However, the exact extent of the disease occurrence in the unorganized herds is not known. The present study was undertaken for serological diagnosis of brucellosis in cattle from a local gaushala (cow shelter) employing common agglutination tests RBPT, STAT and MAT and ELISA. Out of 56 samples from cattle from the Gaushala, 37.5% samples by RBPT, 64.28% each by STAT and MAT and 49.23% samples by ELISA, respectively were found to be positive for anti-Brucella antibodies.