Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle
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Isocitrate dehydrogenase (IDH) catalyzes the reversible conversion between isocitrate and 2-oxoglutarate accom-panied by decarboxylation/carboxylation and oxidoreduc-tion of NAD(P) + cofactor.While this enzyme has beenwell studiedas a catabolic enzyme in the tricarboxylicacid (TCA) cycle, here we have characterized NADP-dependent IDH fromChlorobiumlimicola, agreen sulfur bacteriumthat ®xes CO2 through the reductive tricarboxylic acid (RTCA) cycle, focusing on theCO2-®xation ability of the enzyme. ...
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