Báo cáo khoa học: Cytosol–mitochondria transfer of reducing equivalents by a lactate shuttle in heterotrophic Euglena
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To assess the expression and physiological role of the mitochondrial NAD + -independent lactate dehydrogenase (iLDH) inEuglena gracilis, cells were grown with different carbon sources, and theD-andL-iLDHactivities and several key metabolic intermediates were examined. iLDH activity was significant throughout the growth period, increasing by three- to fourfold from latency to the stationary phase. Intracellular levels ofD-andL-lactate were high (5–40 mM) fromthe start of the culture and increased (20–80 mM)when the stationaryphasewas entered....
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