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Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:8

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Human butyrylcholinesterase (BChE; EC 3.1.1.8) is of particular interest because it hydrolyzes or scavenges a wide range of toxic compounds including cocaine, organophos-phorus pesticides andnerveagents.The relative contribution of each N-linked glycan for the solubility, the stability and the secretionof the enzymewas investigated. Arecombinant monomeric BChE lacking four out of nine N-glycosylation sites and the C-terminal oligomerization domain was stably expressed as a monomer in CHO cells.

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Nội dung Text: Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase

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