Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae
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accharomyces cerevisiaeis a widely used host in the pro-ductionof therapeutic peptides andproteins.Herewe report the identification of a novel endoprotease in S. cerevisiae. It is encoded by the CYM1gene and is specific for the C-terminus of basic residues of heterologously expressed peptides. Gene disruption ofCYM1not only reduced the intracellular proteolysis, but also enhanced the secretion of heterologously expressed peptides such as growth hormone, pro-B-type natriuretic peptide and pro-cholecystokinin....
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