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Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide
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In order to evaluate the ability of the cell-penetrating a-helical amphipathic model peptide KLALKLALKALK AALKLA-NH2 (MAP) to deliver peptide nucleic acids (PNAs) into mammalian cells, MAP was covalently linked to the 12-mer PNA 5¢-GGAGCAGGAAAG-3¢ directed against themRNAof the nociceptin/orphaninFQ receptor. The cellular uptake of both the naked PNA and its MAP-conjugate was studied by means of capillary electrophoresis combined with laser-induced fluorescence detection, confo-cal laser scanningmicroscopyandfluorescence-activatedcell sorting. ...
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