Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli
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Inorder to clarify the role of cytochrome innitrate reductase we have performed spectrophotometric and stopped-flow kinetic studies of reduction and oxidation of the cytochrome hemes with analogues of physiological quinones, using menadione as an analogue of menaquinone and duro-quinone as an analogue of ubiquinone, and comparing the results with those obtained with dithionite. The spectropho-tometric studies indicate that reduction of the cytochrome hemes varies according to the analogue of quinoneused, and in no cases is it complete....
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