Báo cáo khoa học: Fast ferrous heme–NO oxidation in nitric oxide synthases

During catalysis, the heme in nitric oxide synthase (NOS) binds NO before releasing it to the environment. Oxidation of the NOS ferrous heme–NO complex by O2 is key for catalytic cycling, but the mechanism is unclear. We utilized stopped-flow methods to study the reaction of O2 with ferrous heme–NO complexes of inducible and neuronal NOS enzymes.